Human-derived retinoschisis transgenic mice model and construction method thereof

A technology for retinoschisis and transgenic mice, which is applied to other methods of inserting foreign genetic materials, genetic engineering, plant genetic improvement, etc., to achieve the effect of enriching experimental animal subjects

Inactive Publication Date: 2017-10-24
WENZHOU MEDICAL UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

There are no reports on human-derived mouse models at home and abroad, and human-derived mutations RS1 It has not been repor

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  • Human-derived retinoschisis transgenic mice model and construction method thereof
  • Human-derived retinoschisis transgenic mice model and construction method thereof
  • Human-derived retinoschisis transgenic mice model and construction method thereof

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with specific embodiment:

[0022] combined with Figure 1-4 , Example 1, a method for constructing a human-derived retinoschisis transgenic mouse model of the present invention, human-derived RS1 Gene-based screening of human disease-causing genes knocked into mice by gene editing means to obtain the same clinical symptoms as patients RS1h -KI mouse disease model.

[0023] A method for constructing a human-derived retinoschisis transgenic mouse model, using TALENs technology, obtained by screening XLRS patients RS1 For the mutation site of the gene, design the same Oligo sequence as that of the XLRS patient, construct the vector, transfer it into the cell, and screen to obtain the RS1 Stable cell lines with genetic mutations. Express in vitro, isolate mRNA, microinject fertilized eggs and transfer embryos into the uterus of F0 generation, and reproduce to obtain F1 generation with stable phenotyp...

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Abstract

The invention provides a method for constructing a human-derived retinoschisis transgenic mice model. The method comprises the following steps: taking human-derived RS1 genes as basis, screening human pathogenic genes to be knocked in mice through gene editing means, thereby obtaining RS1h-KI mouse disease model.

Description

technical field [0001] The invention relates to a human-derived retinoschisis transgenic mouse model and a construction method thereof. Background technique [0002] Hereditary retinoschisis is an X-chromosomal genetic disease, so it is also called X-linked retinoschisis (XLRS). The disease occurs in whites, blacks and yellow races, with an incidence rate of 1:5000-1:25000. XLRS mainly occurs in males, and it is rare in females. It is more common in children and young adults. It is one of the main genetic diseases that cause blindness in adolescents. The clinical symptoms of XLRS are mostly macular retinoschisis, strabismus, nystagmus, poor vision or even total blindness, ERG is abnormal, b-wave amplitude is significantly reduced, and OCT scan shows obvious "warriform" cyst splitting, severe manifestations Thinning for the thickness of each layer of the retina and the total thickness. To date, there is no effective treatment for hereditary retinoschisis. [0003] RS1 Th...

Claims

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Application Information

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IPC IPC(8): C12N15/89C12N15/877A01K67/027
CPCA01K67/0278A01K2267/0306C12N15/8775C12N15/89
Inventor 谷峰涂孟俊徐陶陈鼎
Owner WENZHOU MEDICAL UNIV
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