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Application of Anti-EpCAM in diagnosis reagent for axillary lymph node metastases of breast cancer and detection method thereof

A technology for lymph node metastasis and breast cancer, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems that cannot be used as a standard for lymph node evaluation of breast cancer, and cannot accurately and accurately judge tumor cells.

Inactive Publication Date: 2017-10-24
PEOPLES HOSPITAL PEKING UNIV
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Problems solved by technology

However, the current non-invasive imaging examinations in clinical practice are all based on morphological changes, so they cannot accurately and accurately judge whether there are a small amount of tumor cells in the lymph nodes.
Therefore, none of these noninvasive tests can be used as a standard for lymph node evaluation in breast cancer

Method used

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  • Application of Anti-EpCAM in diagnosis reagent for axillary lymph node metastases of breast cancer and detection method thereof
  • Application of Anti-EpCAM in diagnosis reagent for axillary lymph node metastases of breast cancer and detection method thereof
  • Application of Anti-EpCAM in diagnosis reagent for axillary lymph node metastases of breast cancer and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1a

[0018] Example 1 Anti-EpCAM / Cy7 and tumor cell-specific binding in vitro experiments

[0019] The VX2 cells in the experimental group were pretreated with anti-EpCAM, and the lymphoid tissues of the control group were pretreated with IgG. After thorough elution, anti-EpCAM / Cy7 was added for cell fluorescence immunoassay. figure 1 The results confirmed that anti-EpCAM / Cy7 can specifically bind to VX2 tumor cells, while normal lymph node tissue has no non-specific binding.

Embodiment 2

[0020] Example 2 Detection of Breast Cancer Axillary Lymph Node Metastasis

[0021] Establishment of breast cancer lymph node metastasis animal model: aseptic resection of the hind leg tumor of VX2 tumor-bearing rabbits, removing the tumor capsule and cutting into fine pieces, grinding and sieving the tissue pieces on a sterile stainless steel mesh, collecting tissue suspension, and sterile normal saline Rinse three times, draw out a 5ml syringe for later use. The tissue suspension was injected into the second pair of breast pads in front of the chest of 12 New Zealand white rabbits in the experimental group, 0.5 ml at each injection site. After the implantation, the tumor growth was detected. After 4 weeks, all implanted tumors survived, with an average diameter of 1.5 cm.

[0022] In vitro fluorescence imaging of axillary lymph nodes: 10umol / L and 0.1mlanti-EpCAM / Cy7 were intradermally injected into the second pair of breasts of 12 tumor-bearing model rabbits and 12 control...

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Abstract

The invention relates to diagnosis for axillary lymph node metastases of breast cancer and particularly relates to an application of Anti-EpCAM in a diagnosis reagent for the axillary lymph node metastases of the breast cancer. The application and the detection method have the advantages that fluorescent dye Cy7-labeled EpCAM monoclonal antibody anti-EpCAM / Cy7 is used for displaying the sensitivity of target development of tumor cells in in-vitro cell experiment, nondestructive detection of the axillary lymph node metastases of the breast cancer can be realized, and the application and the detection method have clinical application value.

Description

technical field [0001] The invention relates to a breast cancer lymph node metastasis detection, in particular to the application of Anti-EpCAM in breast cancer axillary lymph node metastasis detection reagent and kit, and belongs to the field of biological detection reagents. Background technique [0002] Breast cancer is one of the most common malignant tumors in women, and it ranks first in the incidence of female malignant tumors in many large and medium-sized cities in Europe, America and my country. Axillary lymph node metastasis is one of the important prognostic factors of breast cancer, and it also largely determines the decision-making of adjuvant therapy for breast cancer. At present, the clinical evaluation of lymph node metastasis status requires invasive surgery or operations, including axillary lymph node dissection and axillary sentinel lymph node biopsy. For early breast cancer patients without lymph node metastasis, these invasive assessment methods will b...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/574
CPCG01N33/533G01N33/57415
Inventor 王殊郭嘉嘉杨后圃张刘璐
Owner PEOPLES HOSPITAL PEKING UNIV
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