Universal primer and nucleotide sequence of three types of humanized tapeworm ep45 genes as well as identification method of three types of humanized tapeworm

Abstract

The invention relates to the technical field of molecular organisms, and discloses a universal primer and a nucleotide sequence of three types of humanized tapeworm ep45 genes as well as an identification method of three types of humanized tapeworm. The primer comprises an upstream primer disclosed as SEQ ID NO:1 and a downstream primer disclosed as SEQ ID NO:2, wherein the SEQ ID NO:1 is 5'-AGAGACAAAGGAAAGGCAGCTG-3', and the SEQ ID NO:2 is 5'-GGGGCTCAGGCACCTTGAT-3'. According to the implementation way of the invention, the universal primer of the three types of humanized tapeworm ep45 genes is designed, RT-PCR amplification is carried out to obtain the tapeworm ep45 gene segments; then, according to the difference of a restriction enzyme locus among the three types of ep45, EcoR I and/or Aar I are used for carrying out enzyme digestion processing, the three types of humanized tapeworms are interspecific authenticated according to a difference (one/two/three stripes) for enzyme-digested product electrophoresis to generate an atlas, PCR product sequencing authentication time is greatly shortened, and in addition, a judgment result according to an enzyme digestion atlas is more accurate and reliable.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a general primer, nucleotide sequence and identification method of three kinds of human tapeworm ep45 genes. Background technique [0002] Taenia solium, Taenia asiatica and Taenia saginata are important zoonotic parasites, all parasitic in human small intestine, causing human taeniasis and even cysticercosis. Therefore, the identification of species is of great significance in the related research and comprehensive control of taeniasis / cysticercosis. Since the body shapes of the three tapeworms that parasitize the human intestine are very similar, the main difference between the three is whether there are apical processes and small hooks on the scolex. Therefore, the traditional method is to distinguish them through microscopic observation after compression of the scolex. But unfortunately, excreted worms often lack scolex, and it is difficult to identify them solely by proglo...

AI Technical Summary

Problems solved by technology

However, the disadvantage of this method is that sequencing is time-consuming, and when the sequence similarity is very close (hybrid species), accurate inter-species identification cannot be performed

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