A kind of production method of high yield, high selenium Cordyceps militaris

A production method and a technology for Cordyceps militaris, which are applied in directions such as botanical equipment and methods, applications, plant cultivation, etc., can solve problems such as toxicity and difficult absorption by the human body, and achieve the effects of promoting growth and development, low cost, and easy large-scale promotion and production.

Active Publication Date: 2020-08-11
ANKANG AGRI SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If supplemented with inorganic selenium, it is difficult for the human body to absorb, and it has certain toxicity
However, relying solely on selenium in natural foods cannot meet the needs of Chinese residents.

Method used

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  • A kind of production method of high yield, high selenium Cordyceps militaris

Examples

Experimental program
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Effect test

Embodiment 1

[0036] In this embodiment, a method for producing high-yield, high-selenium Cordyceps militaris, the specific process is as follows:

[0037] S1: Cordyceps militaris strain activation

[0038] The PDA solid medium was prepared according to conventional methods, and the medium formula was as follows: 200 g potato, 10 g glucose, 5.0 g sucrose, 5.0 g peptone, 1.0 g potassium dihydrogen phosphate, 0.5 g magnesium sulfate, 12 g agar, and 1000 mL water. The preserved Cordyceps militaris strain was inoculated on the above PDA solid medium, and activated and cultured for 7 days. When the hyphae grew 3 / 4 of the test tube, cultured under light for 12 hours to complete the strain activation of the Cordyceps militaris strain.

[0039] S2: Liquid strain preparation

[0040] The strains activated by the above steps are selected from the test tube strains that have changed color, and the strains are inoculated into the liquid medium for fermentation culture, and the culture time is 5-7 days to obtai...

Embodiment 2

[0051] This example is a method for producing high-yield and high-selenium Cordyceps militaris. The specific process is the same as that of Example 1. The only difference is that in S3, sodium selenite is added in an amount of 150 mg per liter of basal culture solution. The acid is added in an amount of 450 mg per liter of basic culture medium.

Embodiment 3

[0053] This example is a high-yield and high-selenium production method of Cordyceps militaris. The specific process is the same as that of Example 1. The only difference is that in S3, sodium selenite is added at an amount of 200 mg per liter of basal culture solution. The acid was added in an amount of 800 mg per liter of basic culture medium.

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Abstract

The embodiment of the invention discloses a high-yield and high-selenium cordyceps militaris production method, and belongs to the technical field of edible fungus cultivation. The method comprises the steps of cordyceps militaris strain activation, liquid strain preparation, culture medium preparation, inoculation culture, cultivation management achieved in a full-light cultivation and oxygen enrichment cultivation mode and the like. According to the method, sodium selenite serves as a selenium source, so that the cost is low; methionine is added so that the selenium element can be easily absorbed by mycelia; the selenium in a substrate is solidified into cordyceps militaris through metabolism of substances in cordyceps militaris mycelium cells, and the selenium content of cordyceps militaris stromata is greatly increased.

Description

Technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a method for producing cordyceps militaris with high yield and high selenium. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, Cordyceps militaris, belongs to the phylum Eumycota, Ascomycotina, Pyrenomycetes, Clavicipitales, Clavicipitaceae, Cordyceps (Cordyceps), which is the same genus and different species as Cordyceps, is a model species of Cordyceps. A large number of studies have shown that the pharmacological effects of Cordyceps militaris are very similar to those of Cordyceps sinensis. Its medicinal ingredients, cordycepic acid, cordyceps polysaccharide, cordycepin content and SOD activity, are all higher than those of Cordyceps sinensis. Immunity and other functions. [0003] Selenium is one of the essential trace elements for the human body, and is also an internationally recognized "king of anti-cancer eleme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/50A01G18/40A01G18/20
CPCA01G18/00C05B7/00C05C3/00C05C11/00C05D5/00C05D9/02C05F11/00
Inventor 张园园王勇张晶叶岚韩宝康杨柱胡波江新华
Owner ANKANG AGRI SCI RES INST
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