Preparation method of hydrogel with g-quadruplex structure and its application in killing Staphylococcus aureus and Escherichia coli

A quadruplex, hydrogel technology, applied in botanical equipment and methods, applications, biocides, etc., can solve problems such as limiting the clinical application of antibacterial hydrogels, and achieve easy popularization and application, hydrogel stability, and yield. high rate effect

Active Publication Date: 2021-03-16
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] High-quality antibacterial hydrogels require high antibacterial properties, high biocompatibility, and biodegradability, and the raw materials are cheap and easy to obtain, and the preparation is simple. It is difficult to meet the above requirements at the same time, which greatly limits the antibacterial hydrogels clinical application

Method used

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  • Preparation method of hydrogel with g-quadruplex structure and its application in killing Staphylococcus aureus and Escherichia coli
  • Preparation method of hydrogel with g-quadruplex structure and its application in killing Staphylococcus aureus and Escherichia coli
  • Preparation method of hydrogel with g-quadruplex structure and its application in killing Staphylococcus aureus and Escherichia coli

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Embodiment 1

[0031] Example 1: Preparation of antibacterial hydrogel with G-quadruplex structure

[0032] (1) Preparation of benzyloxycarbonyl-L-lysine anhydride (Lys-NCA), the steps are as follows:

[0033] 1) Weigh 5g of L-lysine benzyl ester (purchased) into a 250mL round bottom flask, add 50mL of dry tetrahydrofuran, heat up to 60°C, add 2.5g of triphosgene, and make L-lysine benzyl ester and Sanguang The mol ratio of gas is 2:1, and now triphosgene is excessive;

[0034] 2) Reaction in an oil bath at 60°C for 2 hours, at this time it becomes a clear light yellow solution;

[0035] 3) Concentrate the reaction solution by rotary evaporation, dilute with 5 mL of chloroform, add 250 mL of n-hexane to precipitate under stirring conditions, place it in a refrigerator for cooling and standing for 12 hours, and filter to obtain the crude product;

[0036] 4) Dissolve the above crude product in 20 mL of ethyl acetate at 50°C, filter while hot, gradually add 20 mL of n-hexane to the filtrate ...

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Abstract

The invention relates to a preparation method of a hydrogel having a G-quadruplex structure, and applications of the hydrogel in killing of Staphylococcus aureus and Escherichia coli, wherein a G-quadruplex structure is formed by using guanosine as a main body, and polyethylene glycol-b-polylysine having antibacterial performance is introduced to form the G-quadruplex hydrogel. According to the preparation method, guanosine forms a G-quartet under the stabilization of potassium ions, the vicinal diol of the guanosine and 2-formylbenzeneboronic acid form a dynamic boron ester bond, the primary amine in the polyethylene glycol-b-polylysine having a broad-spectrum antibacterial performance and the aldehyde group of the 2-formylbenzeneboronic acid form a dynamic imine bond, and the G-quartets are connected to form the G-quadruplex through the formations of the boron ester bond and the imine bond so as to form the antibacterial hydrogel. According to the present invention, the preparation method has advantages of simple and easily available raw materials, simple synthesis step, high yield and batch production; and the obtained hydrogel has advantages of stability, low biological toxicity, broad-spectrum antibacterial effect, efficient Gram positive bacterial / Gram negative bacterial killing, and easy promotion and application.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a hydrogel with a G-quadruplex structure used for killing Staphylococcus aureus and Escherichia coli. Background technique [0002] Bacterial infection is an important problem closely related to human health and a major challenge in the field of biomedicine. The design of new antibacterial materials is expected to solve this problem. Hydrogels with antibacterial properties are a class of antibacterial materials with great application value, and have important applications in implanted medical device coatings, wound dressings, etc. [0003] In the prior art, the construction of antibacterial performance of hydrogel can be realized by the following two methods: (1) Physical embedding of antibacterial agents, loading antibacterial agents (such as organic antibiotics, inorganic metal ions, etc.) In hydrogels, the defect of this method is that the antibacterial properties of hydroge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N25/04A01N37/46A01P1/00C07D263/44C08G69/40
CPCA01N25/04A01N37/46C07D263/44C08G69/40
Inventor 马如江李圆凤刘勇史林启安英丽
Owner NANKAI UNIV
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