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Application of TCP1 protein in preparation of postoperative breast cancer prognosis evaluation kit, postoperative breast cancer prognosis evaluation kit and method

A technology for prognostic assessment and breast cancer, applied in the biological field, can solve problems such as unclear molecular mechanism and lack of specific molecular targets for breast cancer treatment

Inactive Publication Date: 2017-11-21
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Breast cancer is one of the most threatening tumors to human health. So far, its molecular mechanism is still unclear, and the treatment of breast cancer also lacks specific molecular targets. As a very important tumor oncogene, TCP1 is currently There is no literature report that TCP1 protein is related to breast cancer prognosis or breast cancer metastasis

Method used

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  • Application of TCP1 protein in preparation of postoperative breast cancer prognosis evaluation kit, postoperative breast cancer prognosis evaluation kit and method
  • Application of TCP1 protein in preparation of postoperative breast cancer prognosis evaluation kit, postoperative breast cancer prognosis evaluation kit and method
  • Application of TCP1 protein in preparation of postoperative breast cancer prognosis evaluation kit, postoperative breast cancer prognosis evaluation kit and method

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Experimental program
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Effect test

Embodiment 1

[0043] A kit for postoperative prognosis assessment of breast cancer, the kit includes: human TCP1 monoclonal antibody or TCP1 polyclonal antibody for detecting the relative expression of TCP1 protein in breast cancer tissue, immunohistochemical experiment reagent. Among them, immunohistochemical reagents include: xylene, ethanol, 3% H 2 o 2 , 3% BSA blocking solution, DAB chromogenic reagent, hematoxylin, horseradish peroxidase, PBS and 0.01M EDTA repair solution.

[0044] The human TCP1 polyclonal antibody of the present invention is obtained by immunizing rabbits with the TCP1 protein whose sequence is shown in SEQ ID NO.1. It can be prepared by itself, or commercially available products can be used.

[0045] Immunohistochemical experiment reagent of the present invention comprises: Xylene, ethanol, 3%H 2 o 2 (aqueous solution), 3% BSA blocking solution (prepared in PBS), DAB chromogenic reagent, hematoxylin, horseradish peroxidase (for labeling secondary antibody), PB...

Embodiment 2

[0047] The application of TCP1 protein in the preparation of a breast cancer postoperative prognosis assessment kit, the application is to use the kit of the present invention to carry out breast cancer postoperative prognosis assessment, the method is as follows:

[0048] (1) Take pathological specimens of breast cancer patients, and use human TCP1 monoclonal antibody or human TCP1 polyclonal antibody, as well as immunohistochemical experimental reagents, for immunohistochemical staining;

[0049] Among them, the pathological specimens come from the biopsy tissue of breast cancer patients or the pathological materials taken during and after the operation. The immunohistochemical method uses the SP staining method, and the specific steps are as follows:

[0050] (a) Paraffin sections of breast cancer tissues were prepared, and the oven was set at 60° C. overnight.

[0051] (b) Dewaxing of slices. Soak in sequence: Xylene I: 10min; Xylene II: 10min; Xylene III: 10min.

[005...

Embodiment 3

[0070] A breast cancer postoperative tumor sample was taken for paraffin-embedded section, and detected by the above-mentioned immunohistochemical method. After calculation, the TCP1 immunohistochemical score of the cancer tissue was 0.586. After postoperative follow-up, it was found that the patient had recurrence and metastasis of breast cancer in the 9th month after the operation, and died 15 months after the operation.

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Abstract

The invention provides the application of the TCP1 protein in the preparation of a breast cancer postoperative prognosis evaluation kit, a breast cancer prognosis evaluation kit and a method. The present invention uses an immunohistochemical method to detect the relative expression of TCP1 protein in breast cancer tissue, so as to judge the risk of breast cancer recurrence and metastasis in breast cancer patients. The beneficial effects of the present invention are mainly reflected in: the present invention provides the application of TCP1 protein in the preparation of breast cancer postoperative prognosis assessment kit, suggesting that the protein can be used to prepare protein molecular markers for judging the prognosis of breast cancer patients. Postoperative monitoring and sequential treatment also have important guiding significance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of TCP1 protein in the preparation of a breast cancer postoperative prognosis evaluation kit, a breast cancer prognosis evaluation kit and a method. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. According to statistics, the incidence rate accounts for 7-10% of all kinds of malignant tumors in the whole body. It is second only to uterine cancer in women's diseases. The incidence of breast cancer is often related to genetics. Patients It is common among women aged 40-60, and the incidence rate of women before and after menopause is higher. Only about 1-2% of breast patients are men. Breast cancer is a malignant tumor that usually occurs in the glandular epithelial tissue of the breast. It is one of the most common malignant tumors that seriously affects women's physical and mental health and even threatens th...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 林尧王清水
Owner FUJIAN NORMAL UNIV
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