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Technology for establishing Pinellia ternate three-generation seed stem breeding system

A three-generation seed and Pinellia technology, applied in the field of establishing a three-generation seed stem breeding system of Pinellia, can solve problems such as provenance degradation, high cost of tissue culture seedlings, and large-scale decay.

Active Publication Date: 2017-11-24
衢州东篱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the harvested pinellia seed stems are generally used in various production areas, or the wild pinellia is excavated to make seeds. The germplasm is easy to degrade and the shape is easy to change. In severe cases, it will cause large-scale rot and a large loss in production. Therefore, it cannot be used continuously. Seed use
[0006] In order to solve the problems of lack of wild provenance, degraded and mixed provenance, high cost of tissue-cultured seedlings and difficulty in being directly used in the production of commercial pinellia, the present invention proposes to establish a three-generation seed stem breeding system, and uses tissue culture technology to solve the degradation problem. At the same time, it overcomes the problem that the price of simple tissue culture seedlings is too high and cannot be applied to commercial production, and effectively solves the problem of provenance

Method used

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  • Technology for establishing Pinellia ternate three-generation seed stem breeding system
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  • Technology for establishing Pinellia ternate three-generation seed stem breeding system

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Embodiment 1

[0040] Embodiment 1, a kind of establishment technique of pinellia pinellia three-generation stem breeding system, comprising:

[0041] Step 1, the cultivation of the original species of pinellia

[0042] Select 2cm-high tissue culture seedlings, cut off the part around the root, take the root 0.2cm×0.2cm×0.2cm, and inoculate it in MS medium + 6-BA0.5mg / L + paclobutrazol activated carbon 0.4mg / L + sucrose 5g / In the medium bottle of L+activated carbon 50mg / L+agar 6.5g / L, the pH of the medium is 5.8, and the temperature is 26±2°C, the light is 2000Lux, and the light is 12 hours / day. Cultivate for 40-50 days. When the diameter of the seed stem in the bottle reaches 0.5-1.0 cm, wash off the surrounding agar, dry the seed stem in the shade, and use it as the original seed for later use.

[0043] Step 2, cultivation of Pinellia original species

[0044]Under the conditions of good natural conditions (high altitude, few aphids, cool climate), good natural isolation conditions, and...

Embodiment 2

[0051] Embodiment 2, a kind of technology for establishing the three-generation stem breeding system of pinellia, comprising:

[0052] Step 1, the cultivation of the original species of pinellia

[0053] Select 2cm-high tissue culture seedlings, cut off the part around the root, take the root 0.2cm×0.2cm×0.2cm, and inoculate it in MS medium + 6-BA0.5mg / L + paclobutrazol activated carbon 0.4mg / L + sucrose 5g / In the medium bottle of L+activated carbon 50mg / L+agar 6.5g / L, the pH of the medium is 5.8, and the temperature is 26±2°C, the light is 2000Lux, and the light is 12 hours / day. Cultivate for 40-50 days. When the diameter of the seed stem in the bottle reaches 0.5-1.0 cm, wash off the surrounding agar, dry the seed stem in the shade, and use it as the original seed for later use.

[0054] Step 2, cultivation of Pinellia original species

[0055] Under the conditions of good natural conditions (high altitude, few aphids, cool climate), good natural isolation conditions, and...

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Abstract

The invention discloses a technology for establishing a Pinellia ternate three-generation seed stem breeding system. The method comprises the following steps: cultivation of Pinellia ternate breeder's seed, cultivation of Pinellia ternate stock seed and cultivation of Pinellia ternate commercial generation seed stem. The viral strain rate of the Pinellia ternate commercial generation seed stem obtained through the method is lower than 5%, and the cost of the commercial generation seed stem is lower than that of ordinary seed steam in the market; the commercial generation seed stem can be in agreement with the market in the aspect of cost, and the acre yield obtained through planting of commercial generation seed stem is extremely higher than that of ordinary Pinellia ternate seed steam in the market.

Description

technical field [0001] The invention belongs to the field of planting Chinese herbal medicines, and in particular relates to a technology for establishing a three-generation seed stem breeding system of pinellia. Background technique [0002] Pinellia (scientific name: Pinellia ternata (Thunb.) Breit) is a perennial herb of the family Araceae. Pinellia is used as medicine with processed underground tubers. The main active ingredients are alkaloids, pinellia protein, etc. Pinellia has the functions of relieving vomiting, resolving phlegm, dispelling swelling and dispelling stagnation. In recent years, it has been found that it has anti-tumor, anti-early pregnancy, liver-protecting, and blood-lipid-lowering effects. Pinellia has the effect of eliminating phlegm in the lungs, which cannot be replaced by other expectorant varieties. [0003] Pinellia resources are widely distributed in my country. Except for Inner Mongolia, Xinjiang, Qinghai, and Tibet, which are not found in...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
CPCA01G24/00A01G31/00A01H4/001A01H4/008
Inventor 徐志明徐诺亚
Owner 衢州东篱生物科技有限公司
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