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Hole punching device and method for efficiently manufacturing plant leaf discs

A technology of punching device and plant leaves, which is applied in metal processing and other directions, can solve the problems of easily contaminated and sterile leaf discs, easy adhesion of leaf discs, and difficulty in dividing into a single tile for cultivation, etc., and achieves the effect of not easy pollution.

Pending Publication Date: 2017-11-24
CHINA TOBACCO YUNNAN IND
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AI Technical Summary

Problems solved by technology

[0004] When using a puncher to make leaf discs, it is easy to contaminate the sterile leaf discs because it is necessary to manually push out the prepared leaf discs with thin iron wires; and when there is a lot of secretions on the surface of the plant leaves, the prepared leaf discs are easy to stick , not easily separated into individual tile cultures

Method used

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  • Hole punching device and method for efficiently manufacturing plant leaf discs

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Embodiment Construction

[0018] The following examples are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased.

[0019] As shown in Figure 1, the punching device for efficiently making plant leaf discs of the present invention is composed of an operating rod 1 and a casing 3, and the spring 5 is put into the cavity of the casing 3, and the lower spring bayonet 6 at the bottom of the casing 3 Clamp the spring, put the operating rod 1 into the casing 3 and pass through the spring 5, the upper spring bayonet 4 clamps the operating rod, hold the operating handle 2 during use, and use the...

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Abstract

The invention provides a hole punching device and method for efficiently manufacturing plant leaf discs. The device comprises an operation rod, an operation handle, a shell, a leaf disc shaping hole, a leaf disc temporary storage cavity and a spring; the operation handle and the shell are connected into one body, the operation rod is arranged in the shell, the spring is arranged on the lower portion of the operation rod, and the operation rod can penetrate through the spring; a leaf disc shaping tube is arranged under the spring, the inner cavity of the tube is the leaf disc temporary storage cavity, and an upper spring bayonet and a lower spring bayonet are arranged on the portions, on the upper portion and the lower portion of the spring, of the shell respectively. The device is used according to a certain application method. By means of the hole punching device and method, the problems that contamination is easy to cause, the adhesion phenomenon easily occurs in the manufacturing process, and the efficiency is low are solved, and a technical support is provided for large-scale genetic transformation of the agrobacterium tumefaciens mediated plants.

Description

technical field [0001] The invention relates to a punching device and method for efficiently manufacturing plant leaf discs. Background technique [0002] The transformation method mediated by Agrobacterium tumefaciens is currently the most widely used plant transgenic method. The transformation method mediated by Agrobacterium tumefaciens is simple to operate, low in cost, high in efficiency, good in insert fragment certainty, and low in transgene copy number. has become the method of choice in transgenic strategies. About 80% of the nearly 200 transgenic plants obtained are mediated by Agrobacterium tumefaciens [Wang Guanlin, Fang Hongyun. Plant Genetic Engineering [M]. Beijing: Science Press, 2002: 452-453]. [0003] During the plant transgenic process mediated by Agrobacterium tumefaciens, in order to improve the transformation efficiency, it is necessary to injure the sterile plant leaves. The current commonly used method is to use a puncher to make plant leaves into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B26F1/32
CPCB26F1/32
Inventor 曾婉俐向海英张建铎高茜张涛李雪梅陈章玉
Owner CHINA TOBACCO YUNNAN IND
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