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Single-layer skin auxiliary material with directional orientation structure and preparation method thereof

A single-layer, skin technology, applied in the field of medical biomaterials, can solve the problems that the pore structure cannot well satisfy the cell migration and growth, the nutrients and wastes cannot be circulated well, and the basic structure cannot be well maintained. Induce skin cell differentiation, facilitate cell adhesion and migration, eliminate necrotic tissue and phagocytose pathogenic bacteria

Active Publication Date: 2020-09-01
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, with people's research on artificial skin accessories, more and more skin accessories have been invented, but the existing preparation methods are too traditional, and their pore structures cannot well meet the migration and growth of cells. Too close together so nutrients and waste don't circulate well
The mechanical properties are poor. During the skin repair period, the auxiliary material structure is easy to collapse, and its basic structure cannot be well maintained, which is not conducive to skin repair
The selection of materials is relatively single, which cannot well meet the various needs in the process of skin repair. Modern medicine believes that the process of skin repair is a complex dynamic process of interaction between various cells, growth factors and extracellular matrix. Each stage has different effects of growth factors, so choosing appropriate materials and growth factors for the skin repair process, and performing targeted repairs can achieve better repair results

Method used

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  • Single-layer skin auxiliary material with directional orientation structure and preparation method thereof
  • Single-layer skin auxiliary material with directional orientation structure and preparation method thereof
  • Single-layer skin auxiliary material with directional orientation structure and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Dissolve a certain amount of sodium alginate powder in absolute ethanol, add a certain amount of deionized water to dilute the mass concentration to 10%, put it in a water bath at 60°C for 6 hours, and store it in a 4°C refrigerator after the powder is completely dissolved. Weigh 1.2g of polylactic acid, 0.45g of collagen and 0.15g of calcium phosphate with an electronic balance, dissolve in 10mL of organic solvent (dichloromethane: ethyl acetate = 7:4), stir for 6h with an electromagnetic stirrer, and mix the polylactic acid and collagen Fully dissolve with calcium phosphate to obtain a uniform and stable polylactic acid / collagen / calcium phosphate mixed solution, add 1.5mL of the prepared sodium alginate solution to the above mixed solution, stir for 6 hours with an electromagnetic stirrer until a homogeneous emulsion, and then Add 100ng of 10ng / mL growth factor, stir for 0.5h to form a uniform and stable spinning solution, draw the spinning solution into the syringe, a...

Embodiment 2

[0032] Dissolve a certain amount of sodium alginate powder in absolute ethanol, add a certain amount of deionized water to dilute the mass concentration to 10%, put it in a water bath at 60°C for 6 hours, and store it in a 4°C refrigerator after the powder is completely dissolved. Weigh 0.8g of polylactic acid, 0.3g of collagen and 0.1g of calcium phosphate with an electronic balance, dissolve in 10mL of organic solvent (dichloromethane: ethyl acetate = 7:4), stir for 6h with an electromagnetic stirrer, and mix polylactic acid, collagen Fully dissolve with calcium phosphate to obtain a uniform and stable polylactic acid / collagen / calcium phosphate mixed solution, add 1mL of the prepared sodium alginate solution to the above mixed solution, use an electromagnetic stirrer to stir for 6h to a homogeneous emulsion, and then add 10ng / mL growth factor 100ng, stirred for 0.5h to form a uniform and stable spinning solution, sucked the spinning solution into the syringe, and used a No. 2...

Embodiment 3

[0034]Dissolve a certain amount of sodium alginate powder in absolute ethanol, add a certain amount of deionized water to dilute the mass concentration to 10%, put it in a water bath at 60°C for 6 hours, and store it in a 4°C refrigerator after the powder is completely dissolved. Weigh 0.64g of polylactic acid, 0.24g of collagen and 0.08g of calcium phosphate with an electronic balance, dissolve them in 10mL of organic solvent (dichloromethane: ethyl acetate = 7:4), stir for 6h with an electromagnetic stirrer, and mix the polylactic acid and collagen Fully dissolve with calcium phosphate to obtain a uniform and stable polylactic acid / collagen / calcium phosphate mixed solution, add 1mL of the prepared sodium alginate solution to the above mixed solution, use an electromagnetic stirrer to stir for 6h to a homogeneous emulsion, and then add 10ng / mL growth factor 100ng, stirred for 0.5h to form a uniform and stable spinning solution, sucked the spinning solution into the syringe, an...

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Abstract

The invention belongs to the technical field of a medical biological material, and particularly relates to a single-layer skin auxiliary material with a directional orientation structure and a preparation method thereof. An electrostatic spinning method is used for preparing a porous reticular single-layer skin auxiliary material containing sodium alginate, collagen, calcium phosphate, lactic acid and growth factors; the porous reticular single-layer skin auxiliary material has the directional orientation structure; the biometric performance is structurally realized; the extracellular matrix structure can be well simulated; the cell adhesion and migration are promoted; the conveyance of nutrition substances and waste materials can be facilitated; the cell growth and proliferation are promoted; meanwhile, the single-layer skin auxiliary material also has certain mechanical property, good biocompatibility and proper degradation performance; the skin cells can be induced to perform proliferation and differentiation; the skin defect can be favorably and effectively restored. The single-layer skin auxiliary material and the preparation method have the advantages that the preparation method is simple; the cost is low; the preparation efficiency is high; huge potential application values are realized.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a single-layer skin auxiliary material with a directional orientation structure and a preparation method thereof. Background technique [0002] Skin injury is a common event in daily life. Skin injury is often caused by construction accidents, fires, daily life accidents and some diseases. It is more common in clinical practice and difficult to treat. According to the degree of injury, it can be divided into minor injury and severe injury. The less damaged area can be repaired by autologous transplantation, but for the larger damaged area and severe injury, the use of autologous transplantation faces a shortage of sources. Secondary surgical injuries and other issues, while large area burns are prone to tissue infection, necrosis and other issues during the repair process. Therefore, it is particularly important to find an alternative skin repair method ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61L27/60A61L27/58A61L27/50A61L27/20A61L27/18A61L27/24A61L27/12A61L27/54D01D5/00
CPCA61L27/12A61L27/18A61L27/20A61L27/24A61L27/50A61L27/54A61L27/58A61L27/60A61L2300/404A61L2300/41A61L2300/414A61L2300/418D01D5/003D01D5/0069D01D5/0076D01D5/0092C08L5/04C08L67/04
Inventor 王欣宇林飞蔡正伟
Owner WUHAN UNIV OF TECH