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Application of timosaponin A-III in preparation of anti-human rhabdomyosarcoma drug

A technology of rhabdomyosarcoma and timosaponin, which can be used in antitumor drugs, drug combinations, pharmaceutical formulations, etc., can solve problems such as no relevant research reports, and achieve the effect of inhibiting proliferation

Active Publication Date: 2017-12-01
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The broad-spectrum and strong anti-tumor activity of TAIII makes it possible to be used in the preparation of drugs for the prevention and treatment of tumors, but there is no relevant research report on the effect of TAIII on rhabdomyosarcoma

Method used

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  • Application of timosaponin A-III in preparation of anti-human rhabdomyosarcoma drug
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  • Application of timosaponin A-III in preparation of anti-human rhabdomyosarcoma drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Effects of timosaponin A-III on the morphology and number of RD in human rhabdomyosarcoma cells observed by microscope

[0037] Experimental materials: human rhabdomyosarcoma cells RD (Rhabdomyosarcoma) were purchased from China Center for Type Culture Collection (CCTCC), Dulbecco's Modified Eagle's Medium (DMEM) high glucose medium and fetal bovine serum (fetal bevine serum, FBS) were purchased from Thermo Fisher Scientific (China) Co., Ltd., 0.25wt% trypsin + 0.02wt% EDTA solution, penicillin-chain Mymycin solution (100×) was purchased from Hangzhou Jinuo Biomedical Technology Co., Ltd. Timosaponin A-III (timosaponin A-III, TAIII) was purchased from Dalian Meilun Biotechnology Co., Ltd., dimethyl sulfoxide (dimethyl sulfoxide , DMSO) were purchased from Sigma-AldrichCo.LLC.

[0038] The RD cell culture medium is a DMEM high-glucose medium supplemented with 10% FBS by volume and 1% penicillin-streptomycin solution (100×) by volume. Prepare timosaponin A-III with DMS...

Embodiment 2

[0042] The half maximal inhibitory concentration (IC) of timosaponin A-III and the reference compound vincristine sulfate on the proliferation of human rhabdomyosarcoma cells RD was determined by MTT method. 50 )

[0043] Experimental materials: human rhabdomyosarcoma cells RD (Rhabdomyosarcoma) were purchased from China Center for Type Culture Collection (CCTCC), Dulbecco's Modified Eagle's Medium (DMEM) high glucose medium and fetal bovine serum (fetal bevine serum, FBS) were purchased from Thermo Fisher Scientific (China) Co., Ltd., 0.25wt% trypsin + 0.02wt% EDTA solution, penicillin-chain Mymycin solution (100×) was purchased from Hangzhou Jinuo Biomedical Technology Co., Ltd., and thiazolyl blue was 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (3 -(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, MTT) was purchased from Sangon Bioengineering (Shanghai) Co., Ltd. Timosaponin A-III (timosaponin A- III, TAIII), vincristine sulfate (vincristine...

Embodiment 3

[0050] Effect of timosaponin A-III on apoptosis of human rhabdomyosarcoma cells detected by Annexin V-FITC / PI double staining flow cytometry

[0051]Experimental materials: human rhabdomyosarcoma cells RD (Rhabdomyosarcoma) were purchased from China Center for Type Culture Collection (CCTCC), Dulbecco's Modified Eagle's Medium (DMEM) high glucose medium and fetal bovine serum (fetal bevine serum, FB) were purchased from Thermo Fisher Scientific (China) Co., Ltd., 0.25wt% trypsin + 0.02wt% EDTA solution, penicillin-chain Mymycin solution (100×) was purchased from Hangzhou Jinuo Biomedical Technology Co., Ltd. Timosaponin A-III (timosaponin A-III, TAIII) was purchased from Dalian Meilun Biotechnology Co., Ltd., dimethyl sulfoxide (dimethyl sulfoxide , DMSO) was purchased from Sigma-AldrichCo.LLC, and the Annexin V-FITC / PI apoptosis detection kit was purchased from Dongren Chemical Technology (Shanghai) Co., Ltd.

[0052] The RD cell culture medium is a DMEM high-glucose medium...

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Abstract

The invention discloses an application of timosaponin A-III in preparation of anti-human rhabdomyosarcoma drug, and belongs to the technical field of new medicine purpose of timosaponin A-III. The key point of the technical scheme is that the application of timosaponin A-III in preparation of anti-human rhabdomyosarcoma drug, wherein the molecular formula of the timosaponin A-III is C39H64O13; the chemical structural formula is as shown in specification. The timosaponin A-III can effectively inhibit the proliferation of human rhabdomyosarcoma RD; the median inhibitory concentration of the treated RD cell for 24 h is 4.28 mu m, and is about 1 / 22 of the contrast compound vincristine sulfate IC 50 (92.86 mu m). The 10 mu M timosaponin A-III can induce the significant apoptosis of the human rhabdomyosarcoma, which indicates that the timosaponin A-III can effectively inhibit the proliferation of human rhabdomyosarcoma, and is expected to be developed to be the effective component of the human rhabdomyosarcoma.

Description

technical field [0001] The invention belongs to the technical field of new medical applications of timosaponin A-III, and specifically relates to the application of timosaponin A-III in the preparation of anti-human rhabdomyosarcoma drugs. Background technique [0002] Rhabdomyosarcoma (rhabdomyosarcoma, RMS) is a soft tissue malignant tumor originating from the mesenchyme, which is the most common type of soft tissue sarcoma in children, accounting for about 3.5% of cancer cases in children aged 0-14 years, and accounting for cancer cases in adolescents aged 15-19 years. 2% of [1-2] . The incidence of RMS is 4.5 cases per million children, with 50% of cases presenting before the age of 10 [1] . At present, RMS adopts a comprehensive treatment based on surgery, chemotherapy and radiotherapy. [2] . All children with RMS need to receive chemotherapy, the standard chemotherapy regimen is VAC-vincristine (vincristine), actinomycin (actinomycin-D), cyclophosphamide (cyclopho...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P35/00
CPCA61K31/7048
Inventor 陶玲段广才谷玉梅刘影影王伟李光飞
Owner XINXIANG MEDICAL UNIV
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