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Method for producing water-soluble micro-ecological preparation for poultry by virtue of arginine overflow liquid

A micro-ecological preparation, arginine technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as not much research, and achieve the effects of good application effect, rich nutrition and simple control.

Pending Publication Date: 2017-12-08
辽宁威兰生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is really not much research on Lactobacillus acidophilus and arginine in amino acids as a community

Method used

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  • Method for producing water-soluble micro-ecological preparation for poultry by virtue of arginine overflow liquid
  • Method for producing water-soluble micro-ecological preparation for poultry by virtue of arginine overflow liquid
  • Method for producing water-soluble micro-ecological preparation for poultry by virtue of arginine overflow liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Preparation of arginine overflow liquid: arginine is extracted from the arginine overflow liquid according to (Chemical Industry Press Biological L-Glutamine and L-Arginine Fermentation Production by Li Wenlian, pp. 162-168) The liquid after that is referred to in the present invention as arginine excess liquid.

[0041] Gained arginine overflow liquid comprises (weight parts): protein (dry basis) 50.0%, and amino acid content is (dry basis) 39.5%; In amino acid, comprises (dry basis): lysine 3.8%, methionine 2.2%, tryptophan 1.2%, cystine 1.2%, arginine 5.2%, glutamic acid 7.8%, glycine 6.9%, leucine 8.8%; and small peptides below 2000 daltons (dry basis ) content: 32.0%; the solid content in the arginine overflow liquid is 10%.

[0042] (2) Preparation of Lactobacillus acidophilus seed liquid:

[0043] 1) Single colony isolation: spread the activated Lactobacillus acidophilus slant strain on a petri dish filled with newly configured glucose-peptone beef extract ...

Embodiment 2

[0048]The arginine overflow liquid obtained in Example 1 is used to test the impact of the water-soluble probiotics on the egg production rate and feed-to-egg ratio of Hailan brown chickens, adding it to the drinking water of 28-week-old Hailan brown laying hens. The amount added is 5000ML for each group of test chickens per day, that is, 10ML / day. The test results of the production of water-soluble probiotics for poultry with arginine overflow liquid on the egg production rate and feed-to-egg ratio of Hailan brown chickens are shown in the table. 1:

[0049] Table 1. Production of poultry water-soluble probiotics by arginine overflow liquid

[0050] Effects on Laying Rate and Feed-egg Ratio of Hai-Line Brown Chicken

[0051]

[0052] Note: The number of experimental animals is 500 per group; the experimental period is 30 days.

[0053] It can be seen from Table 1 that the production of poultry water-soluble probiotics by arginine overflow liquid has an effect on the egg ...

Embodiment 3

[0055] Add the water-soluble probiotics for poultry produced by the arginine overflow liquid obtained in Example 1 to the drinking water of 42-day-old broilers. The test results of the impact of the water-soluble probiotics on the average daily gain and feed-to-meat ratio of broiler chickens in the production of poultry water-soluble probiotics from acid overflow liquid are shown in Table 2:

[0056] Table 2 Production of poultry water-soluble probiotics by arginine overflow liquid

[0057] Effects on average daily gain and feed-to-meat ratio of broilers

[0058]

[0059] Note: The number of experimental animals is 500 per group; the age is 42 days; the test period is 30 days;

[0060] It can be seen from Table 2 that the water-soluble probiotics for poultry produced by arginine overflow liquid was added to the drinking water of 42-day-old broilers, and the daily gain increased significantly, and the feed-to-egg ratio decreased. Compared with the control group, the test gr...

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Abstract

The invention discloses a method for producing a water-soluble micro-ecological preparation for poultry by virtue of arginine overflow liquid, and relates to the fields of amino acid technology and microbial fermentation technology. With lactobacillus acidophilus serving as an original strain, and the arginine overflow liquid, glucose, sodium chloride and magnesium sulfate as mediums, the micro-ecological preparation for poultry, which is water-soluble, functional and suitable for drinking, is prepared by conducting single-colony separation and purification on the lactobacillus acidophilus, preparation of the arginine overflow liquid, liquid seed culture and liquid fermentation culture. According to the method provided by the invention, the lactobacillus acidophilus strain is adopted as a culture strain and the adopted medium consists of the arginine overflow liquid, the glucose, the sodium chloride and the magnesium sulfate. The finished product (the micro-ecological preparation), as water-soluble liquid, is rich in nutrition, good in effects of preventing and treating diseases and obvious in effect of promoting growth when applied to breeding of the poultry; and the finished product can improve quality and can also improve a breeding environment; therefore, the finished product is the green and high-quality micro-ecological preparation for poultry, which is water-soluble, functional and suitable for drinking.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation of amino acid production technology, in particular to a method for producing poultry water-soluble probiotics by using arginine overflow liquid. Background technique [0002] Arginine is a kind of amino acid family. Like other amino acids, arginine has the function of maintaining the growth and development of living organisms, metabolism and other life activities. Arginine is a conditionally essential amino acid. In recent years, people's research on arginine has been perfected day by day. Its main function is to maintain the nitrogen balance of amino acid species, promote and maintain normal growth and development, improve and regulate immune function, promote wound healing, promote the synthesis of collagen tissue, and repair wounds, etc. . [0003] In recent years, arginine has been widely used as an auxiliary drug in the treatment of human diseases, and has also been widely us...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A61K35/747A61P3/00A61P37/04A61P3/02C12R1/23
CPCA23K10/18A61K35/747C12N1/20A23V2400/113
Inventor 李铁军齐玉艳
Owner 辽宁威兰生物技术有限责任公司
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