An analytical method for detecting catalase activity in textile printing and dyeing industry

Abstract

The invention relates to an analysis method for detecting the activity of catalase in the textile printing and dyeing industry, which comprises the following steps: (1) diluting the enzyme dilution liquid to be tested obtained by diluting the buffer solution and adding the enzyme dilution solution into multiple graduated test tubes or colorimetric tubes; Hydrogen oxide substrate solution was incubated at 30±10°C for 3 to 10 minutes; (2) Add acid solution to one of the tubes first, then add the dilution of the enzyme to be tested, as a blank, add the enzyme to be tested to the other tubes first Diluent, after reacting for 3 to 10 minutes, add acid solution immediately to stop the enzyme action; (3) Add titanium solution to the tube, constant volume, shake well, and after standing still, measure the absorbance of the samples in each tube at 400 to 450 nm (4) Calculate the enzyme activity of catalase according to the measured absorbance in conjunction with the calibration curve. Compared with the prior art, the present invention does not have high requirements on the concentration accuracy of hydrogen peroxide during the test, but has high test result accuracy, high test efficiency and simple operation.

Description

technical field [0001] The invention relates to an analysis method for the activity of catalase, in particular to an analysis method for detecting the activity of catalase in the textile printing and dyeing industry. Background technique [0002] Catalase, a biological enzyme that catalyzes the decomposition of hydrogen peroxide into oxygen and water, exists in the peroxide body of cells. Catalase is used in the textile printing and dyeing industry to remove residual hydrogen peroxide from the oxygen bleaching process, which reduces the waste of water resources caused by washing with a large amount of water in the traditional process, and also reduces the waste water containing hydrogen peroxide from entering the sewage The stress that the treatment system puts on the sewage system. [0003] The iodometric method is commonly used to measure the enzyme activity of catalase. The iodometric method is to generate iodine through the reaction of hydrogen peroxide and iodide ions,...

AI Technical Summary

Problems solved by technology

This method has the following three disadvantages: first, the titration experiment requires a higher technical level of the operator, and it needs to be operated by an operator trained for a long time, otherwise the accuracy of the experimental result is difficult to guarantee

Second, using starch solution as an indicator, at the titration end point, the reaction to generate a light blue complex is slow, so a single titration takes a long time

Third, a single titration takes a long time, so that only one sample can be tested at a time, so when there are more samples to be tested, the test time will increase exponentially

Fourth, the stability of hydrogen peroxide becomes worse after it is dilute, and it will decompose naturally after being placed for a long time, so the later the test, the greater the system error

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