An analytical method for detecting catalase activity in textile printing and dyeing industry

A catalase, textile printing and dyeing technology, applied in the direction of material analysis by optical means, analysis of materials, preparation of test samples, etc., can solve the problems of large system error, increased test time, and high requirements for operators' technical level. , to achieve high accuracy and repeatability, reduce system errors, and save test time.

Active Publication Date: 2021-02-05
FIBER CHEM BIOLOGICAL CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following three disadvantages: first, the titration experiment requires a higher technical level of the operator, and it needs to be operated by an operator trained for a long time, otherwise the accuracy of the experimental result is difficult to guarantee
Second, using starch solution as an indicator, at the titration end point, the reaction to generate a light blue complex is slow, so a single titration takes a long time
Third, a single titration takes a long time, so that only one sample can be tested at a time, so when there are more samples to be tested, the test time will increase exponentially
Fourth, the stability of hydrogen peroxide becomes worse after it is dilute, and it will decompose naturally after being placed for a long time, so the later the test, the greater the system error

Method used

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  • An analytical method for detecting catalase activity in textile printing and dyeing industry
  • An analytical method for detecting catalase activity in textile printing and dyeing industry
  • An analytical method for detecting catalase activity in textile printing and dyeing industry

Examples

Experimental program
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Effect test

Embodiment 1

[0040] An analytical method for detecting catalase activity in the textile printing and dyeing industry, comprising the following steps:

[0041] (1) Drawing of calibration curve

[0042] Accurately draw 0.00mL, 0.25mL, 0.50mL, 0.70mL, 1.00mL, 2.5mL, 5.00mL, 7.00mL and 10.00mL of 20mg / L hydrogen peroxide standard solution into a 25mL stoppered colorimetric tube, add 5mL concentration It is 5g / L titanium dioxide-sulfuric acid solution, dilute to 25mL, shake well, after standing for 10min, measure the absorbance at 430nm with a 1cm cuvette;

[0043] According to the absorbance and the corresponding hydrogen peroxide content, draw a calibration curve.

[0044] (2) Determination of samples

[0045] (2-1) Add 5mL, 20mg / L hydrogen peroxide substrate solution in three 25mL colorimetric tubes, dilute the enzyme solution to be tested with pH=7.0 buffer solution to obtain the enzyme dilution solution to be tested, Put the hydrogen peroxide substrate solution and the enzyme dilution t...

Embodiment 2

[0064] The analytical method of catalase activity in the detection textile printing and dyeing industry of the present embodiment is basically the same as that of Example 1, the difference is that the H added in the step (2-2) and the step (2-3) of the present embodiment 2 SO 4 The volume is 2mL.

Embodiment 3

[0066] The analytical method of catalase activity in the detection textile printing and dyeing industry of the present embodiment is basically the same as that of Example 1, the difference is that the H added in the step (2-2) and the step (2-3) of the present embodiment 2 SO 4 The volume is 7mL.

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Abstract

The invention relates to an analysis method for detecting the activity of catalase in the textile printing and dyeing industry, which comprises the following steps: (1) diluting the enzyme dilution liquid to be tested obtained by diluting the buffer solution and adding the enzyme dilution solution into multiple graduated test tubes or colorimetric tubes; Hydrogen oxide substrate solution was incubated at 30±10°C for 3 to 10 minutes; (2) Add acid solution to one of the tubes first, then add the dilution of the enzyme to be tested, as a blank, add the enzyme to be tested to the other tubes first Diluent, after reacting for 3 to 10 minutes, add acid solution immediately to stop the enzyme action; (3) Add titanium solution to the tube, constant volume, shake well, and after standing still, measure the absorbance of the samples in each tube at 400 to 450 nm (4) Calculate the enzyme activity of catalase according to the measured absorbance in conjunction with the calibration curve. Compared with the prior art, the present invention does not have high requirements on the concentration accuracy of hydrogen peroxide during the test, but has high test result accuracy, high test efficiency and simple operation.

Description

technical field [0001] The invention relates to an analysis method for the activity of catalase, in particular to an analysis method for detecting the activity of catalase in the textile printing and dyeing industry. Background technique [0002] Catalase, a biological enzyme that catalyzes the decomposition of hydrogen peroxide into oxygen and water, exists in the peroxide body of cells. Catalase is used in the textile printing and dyeing industry to remove residual hydrogen peroxide from the oxygen bleaching process, which reduces the waste of water resources caused by washing with a large amount of water in the traditional process, and also reduces the waste water containing hydrogen peroxide from entering the sewage The stress that the treatment system puts on the sewage system. [0003] The iodometric method is commonly used to measure the enzyme activity of catalase. The iodometric method is to generate iodine through the reaction of hydrogen peroxide and iodide ions,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/38
CPCG01N1/38G01N21/31G01N2021/3188
Inventor 蒋秀玲吴浩郑慧春欧乐登陈红新
Owner FIBER CHEM BIOLOGICAL CHEM
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