A device and method for using microalgae to treat ammonia nitrogen wastewater
An ammonia nitrogen wastewater and microalgae technology, applied in enzymology/microbiology devices, biomass post-processing, chemical instruments and methods, etc., can solve the problem of inability to be used repeatedly for a long time, decreased pellet strength, and low ammonia nitrogen concentration and other problems, to improve the removal rate of ammonia nitrogen and the ability of microalgae to treat ammonia nitrogen, and to achieve the effect of repeated use
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[0067] 1. Materials
[0068] Microalgae strains and culture conditions: select a single colony of Chlorella vulgaris growing on the surface of the agar dish 21, and transfer the microalgae colony to the culture solution prepared in advance and sterilized by a high-pressure steam sterilizer.
[0069] The culture medium used was 3N-BBM+V culture medium, in which 0.2g / L glucose was added, and the formula of 3N-BBM+V culture medium was as follows (concentration units are all g / L): NaNO 3 ,0.75; CaCl 2 2H 2 O,0.025; MgSO 4 ·7H 2 O,0.075;K 2 HPO 4 ·3H 2 O,0.075; KH 2 PO 4 , 0.175; NaCl, 0.025. In addition, the culture fluid also contains other trace components, including (concentration units are mg / L): Na 2 EDTA, 4.5; FeCl 3 ·6H 2 O,0.582; MnCl 2 4H 2 O,0.246; ZnCl 2 ,0.03; CoCl 2 ·6H 2 O, 0.012; Na 2 MoO 4 2H 2 O,0.024; VB 1 ,1.2; VB 12 ,0.01. 20 mL of culture broth was placed in a 25 mL flask 22 .
[0070] The culture solution containing Chlorella was cultu...
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