A strain of Bacillus amyloliquefaciens and its application in degrading feathers to produce oligopeptides

A technology for dissolving amyloid spores and bacilli, which is applied in the field of microorganisms, can solve the problems of industrial production, difficulty in full utilization and rapid consumption, and high content of macromolecular protein, which are unfavorable for the regeneration and utilization of livestock and poultry waste, and achieve full recycling and low feather weight. The effect of high peptide content and optimal feed efficiency

Active Publication Date: 2020-04-03
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But in fact, due to the high content of macromolecular protein in fermented products and low digestibility, the amount that can be added in animal feed is less than 10%, resulting in a lot of waste of resources
However, the yield of oligopeptides in the fermentation product of feather keratin degradation, especially in the application of animal feed, has not received much attention, which has led to a large amount of renewable resources obtained by efficient degradation fermentation, which is difficult to be fully utilized and Rapid consumption, resulting in a backlog of renewable resources, is not conducive to the realization of industrial production of livestock and poultry waste recycling

Method used

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  • A strain of Bacillus amyloliquefaciens and its application in degrading feathers to produce oligopeptides
  • A strain of Bacillus amyloliquefaciens and its application in degrading feathers to produce oligopeptides
  • A strain of Bacillus amyloliquefaciens and its application in degrading feathers to produce oligopeptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the screening of bacterial strain

[0031] 1. Enrichment and acclimatization: Take a mixed sample of soil that has accumulated feathers for a long time, fermentation pond residue and sewer sludge, and mix it with water at a mass ratio of 1:5. Take 10ml of it and put it into a 250ml Erlenmeyer flask filled with 80ml No. 1 enrichment medium, cultivate it on a shaker at 37°C, pH value 7, and 150r / min until the feathers are basically rotted; then take the enrichment medium in the bottle The liquid was inoculated into No. 2 enrichment medium at an inoculum amount of 5%, cultivated under the same conditions as above until the feathers were basically rotted, and passed twice.

[0032] The composition of the No. 1 enrichment medium is: NH 4 Cl 0.5g, NaCl 0.5g, K 2 HPO 4 0.7g, KH 2 PO 4 0.35g, MgSO 4 ·7H 2 O 0.2g, yeast extract 0.1g, glucose 1g, pH value 7.0, add feather 5g, sterilize at 115°C for 30min.

[0033] The composition of the No. 2 enrichment med...

Embodiment 2

[0040] Embodiment 2: bacterial strain identification

[0041] 1. Observation of bacterial colony and bacterial morphology: The purified and isolated Bacillus amyloliquefaciens H5 was cultured by streaking on the beef extract peptone plate medium, and cultured at a constant temperature of 37°C for 48 hours. The colonies were observed to be nearly round, flat, and centered by naked eyes. Raised, with nicks on the edges, opaque, sticky and hard to pick. Observed under a scanning electron microscope, as figure 1 As shown, the bacterium was rod-shaped, 1.12-2.34 μm long, without flagella, and the Gram test was positive (such as figure 2 shown).

[0042] 2. Observation of culture characteristics: Bacillus amyloliquefaciens H5 was inoculated in beef extract peptone medium with the same inoculum amount, cultured on a shaking table at different temperatures for 18 hours, and the strains in the environment of 15-50°C could grow, and The strain grows fastest in the environment of 28-...

Embodiment 3

[0045] Example 3: Optimization of fermentation conditions for producing oligopeptides

[0046] 1) Preparation of secondary seed liquid: Pick a ring of Bacillus amyloliquefaciens H5 preserved on a slant, inoculate it into beef extract peptone test tube culture medium at 28-40°C for 12 hours to obtain primary seed liquid, and then inoculate the primary seed liquid according to Inoculate 2% of the inoculum into 30ml beef extract peptone medium and incubate at 28-40°C for 12h to obtain the secondary seed solution.

[0047] The beef extract peptone medium comprises: peptone 10g / L, beef extract 5g / L, sodium chloride 5g / L, pH value 6.0-8.0, sterilized at 121°C for 20min.

[0048] 2), fermentation and degradation of feathers: the secondary seed liquid of Bacillus amyloliquefaciens H5 was inserted into the fermentation medium at an inoculum size of 2%, and a single factor experiment was used to investigate the degradation rate of feathers by different fermentation pH, fermentation temp...

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Abstract

The invention relates to the technical field of microorganisms. The purpose is to provide a strain of Bacillus amyloliquefaciens H5 with high yield of oligopeptides in fermentation products, and the application of the strain in degrading feathers to produce feather oligopeptide powder. The deposit number of Bacillus amyloliquefaciens H5 is: CGMCC No.14264. The technical scheme adopted in the present invention is: carry out activation culture to bacillus amyloliquefaciens H5, obtain the primary seed liquid, then inoculate the primary seed liquid in the seed culture medium and cultivate to obtain the secondary seed liquid; inoculate the secondary seed liquid in Cultured in fermentation medium, the obtained fermentation product can be used to prepare feather oligopeptide powder or oligopeptide solution. The invention can almost completely degrade the whole feather, and the yield of the feather oligopeptide in the degradation product is high, and can be used to produce the feather oligopeptide powder which is easy to digest and has high added value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Bacillus amyloliquefaciens and the application thereof to produce oligopeptides by degrading feathers. Background technique [0002] In recent years, with the continuous expansion of the scale of poultry breeding in my country, a large amount of livestock waste, such as feathers, hair, horns, hooves, etc., has been produced, and the annual output of discarded feathers has reached hundreds of thousands of tons, causing serious environmental burdens. The analysis results show that the main component of feathers is keratin, and its crude protein content can reach more than 80%, and it contains a variety of amino acids, as well as many major elements, trace elements and some unknown growth factors. It is a potential value. High dietary protein source. However, due to the existence of a large number of disulfide bonds, hydrogen bonds and hydrophobic gro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00C12R1/07
CPCC12P21/00C12N1/205C12R2001/07
Inventor 李东黄艳蒙刘晓风廖银章曹沁
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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