Lyophilized platelet rich plasma for the use in wound healing (chronic or acute) and bone or tissue grafts or repair

Abstract

This invention relates to an improved Lyophilized platelet rich plasma used to make a platelet gel wound healant, and methods of preparation and use thereof for healing wounds are disclosed. The improved wound healant comprises therapeutically effective amounts of activated growth factors, platelet ghost, plasma (know as the plasma back bone), white blood cells with optional none, one or more additional anti-oxidant such as vitamin A and / or C and / or E, and / or none one or more antibiotics and / or GHK-Cu (produced by ProCyte Inc.)

Description

FIELD OF TH INVENTION [0001] This application is based on Provisional application No. 60 / 542,903 Filed Feb. 9, 2004. The invention relates to an improved Lyophilized platelet rich plasma used to make a platelet gel wound healant, and methods of preparation and use thereof for healing wounds are disclosed. The improved wound healant comprises a therapeutically effective amount of activated growth factors with optional none, one or more additional anti-oxidant such as vitamin A and / or C and / or E, and / or one or more antibiotics and / or GHK-Cu (produced by ProCyte Inc.). [0002] The present invention relates to improved Lyophilized platelet rich plasma for the use in wound healing and bone or tissue grafts and methods of making and use thereof of Lyophilized or fixed platelets. [0003] The present invention generally relates to the therapeutic uses of blood platelets and fresh plasma that has been combined and lyophilized, and more particularly to manipulations or modifications of platelet...

AI Technical Summary

Benefits of technology

"The present invention provides a dehydrated composition of freeze-dried platelets and fresh plasma that can be preserved for storage and rehydration. The composition can also contain other agents such as antibiotics, antifungals, growth factors, or the like, for therapeutic applications. The composition can be applied to wounds to promote healing and prevent infection. The invention also describes a method of making the dehydrated composition and the use of the composition for wound treatment. The invention expands the uses of concentrated platelet materials and enhances their performance by improving their biological properties and making them more shelf stable. The invention also involves adding antibiotics and vitamins to the concentrated platelet gel to enhance its wound healing and anti-oxidant properties."

Problems solved by technology

However, a considerable fraction of these cells are partly lysed after thawing and have the shape of a balloon.

Proper functioning of lyophilized platelets that have been fixed by such fixative agents in hemostasis is questionable.

However, electroporation is very damaging to the cell membranes and is believed to activate the platelets.

Activated platelets have dubious clinical value.

Although injury to the surface of the skin is the most well known type of wound, the surfaces of internal organs may also be wounded, such as during surgery, rupture of the spleen or liver, or resulting from traumatic blows to the body surface in the vicinity of an internal organ.

Acute wounds covering a large or movable surface are usually the most difficult to guard from infection, and to heal.

These products form a hard, cast-like covering over the area to be sealed, and tend to be non-yielding to limb movement.

This form of treatment has major problems, like time consuming and problems collecting blood sometimes with expensive machines and disposables.

The chemical reactions and cascades that normally occur when thrombin is added to the concentrated platelets are indeed complex.

There are a number of disadvantages associated with conventional wound compositions derived from platelet concentrates.

Also, certain platelet compositions must be applied to the wound site on a daily basis and thus require regular blood withdrawal from the patient.

This is time consuming and if more of the compositions is needed, then another needle stick is needed and the process time required to make the composition.