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Composition for enhancing immunity including ginsenoside f1 as active ingredient

A ginsenoside and immune-enhancing technology are applied in the fields of immune-enhancing pharmaceutical compositions, perforin or granzyme preparation compositions, and immune-enhancing food compositions, and can solve problems such as difficulty in separating rare ginsenosides.

Inactive Publication Date: 2017-12-29
INTELLIGENT SYNTHETIC BIOLOGY CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the ginsenosides, rare ginsenosides present in ginseng in very small amounts are difficult to isolate, and thus have not been studied

Method used

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  • Composition for enhancing immunity including ginsenoside f1 as active ingredient
  • Composition for enhancing immunity including ginsenoside f1 as active ingredient
  • Composition for enhancing immunity including ginsenoside f1 as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1: Degranulation activity effect of innate immune cells produced by ginsenoside F1

[0080] The degree of degranulation activity of innate immune cells produced by ginsenoside F1 was determined by flow cytometric analysis (fluorescence activated cell sorter, FACS).

[0081] Specifically, after pretreating peripheral blood mononuclear cells (peripheral blood mononuclear cells, PBMCs) isolated from blood and natural killer cells isolated with ginsenoside, the above cells were combined with K562 cells or 721.221 cells as target cells, respectively. to cultivate. Afterwards, the surface of peripheral blood mononuclear cells and natural killer cells was stained using fluorochrome-conjugated anti-CD3, anti-CD56 and anti-CD107a antibodies. On the other hand, if figure 1 As shown, the degranulation activity of peripheral blood mononuclear cells or natural killer cells is proportional to the expression of CD107a on the cell surface, so the expression of CD107a in peri...

Embodiment 2

[0084] Example 2: Cell killing activity effect of natural killer cells produced by ginsenoside F1

[0085] In order to confirm whether ginsenoside F1 enhances the cell killing activity of natural killer cells, the degree of cytolysis of target cells was measured.

[0086] Specifically, after pretreatment with ginsenoside F1 or Rg3, peripheral blood mononuclear cells (peripheral blood mononuclear cells, PBMC) isolated from blood and simply isolated natural killer cells were mixed with europium-labeled K562 cells or 721.221 cells as target cells were cultured together. The aforementioned natural killer cells and target cells were mixed and cultured at ratios of 5:1, 10:1, 20:1 and 40:1. On the other hand, the degree of fluorescence released from target cells is proportional to the degree of cell-killing activity of natural killer cells. Therefore, after centrifuging the above-mentioned culture solution, use a microplate reader to measure the degree of cell lysis caused by targe...

Embodiment 3

[0088] Example 3: Effect of cell killing activity of primary natural killer cells produced by ginsenoside F1

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Abstract

The present invention relates to composition for enhancing immunity, comprising ginsenoside F1 as an active ingredient. Specifically, the composition according to the present invention promotes degranulation activity and cell-killing activity of natural killer cells, and increases expressions of cell-killing factors, thereby being effectively used as an immune enhancer.

Description

technical field [0001] The present invention relates to a pharmaceutical composition for enhancing immunity comprising ginsenoside F1 as an active ingredient. [0002] Moreover, this invention relates to the food composition for immunity enhancement containing ginsenoside F1 as an active ingredient. [0003] Furthermore, the present invention relates to an immunoenhancing method comprising the step of administering ginsenoside F1 to an individual. [0004] Furthermore, the present invention relates to a composition for preparing perforin or granzyme comprising ginsenoside F1 and natural killer cells. Background technique [0005] Immunity is roughly divided into innate immunity (innate immunity) possessed after birth, and acquired immunity (acquired immunity) acquired congenitally by adapting to life and the like. Among them, innate immunity, also known as "natural immunity", reacts non-specifically with antigens and does not display special memory functions. Such innate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7032A61K31/704A61K35/17A23L33/105A61P37/04
CPCA23V2002/00A61K31/7032A61K31/704A61K35/17A01N2300/00A23V2250/2124A23V2200/324A23L33/105A61P37/00A61P37/04A61P43/00C12N9/6467C12Y304/21079A23L33/10C07K14/47
Inventor 金善昌金宪湜
Owner INTELLIGENT SYNTHETIC BIOLOGY CENT
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