Targeting inhibitor for MCM3AP-AS1 gene and application of targeting inhibitor

A technology of MCM3AP-AS1 and inhibitors, which is applied in the field of medicine, can solve the problems of blank application of MCM3AP-AS1, and achieve the effect of no adverse reactions, reducing drug resistance and definite therapeutic effect

Active Publication Date: 2017-12-29
SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] At present, the role and related mechanism of MCM3AP-AS1 in the development of glioma have not been reported, and the application of MCM3AP-AS1 in glioma gene therapy is still blank.

Method used

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  • Targeting inhibitor for MCM3AP-AS1 gene and application of targeting inhibitor
  • Targeting inhibitor for MCM3AP-AS1 gene and application of targeting inhibitor
  • Targeting inhibitor for MCM3AP-AS1 gene and application of targeting inhibitor

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Embodiment 1

[0039] 1. Glioma vascular endothelial cell culture and preparation of conditioned medium.

[0040] The human brain microvascular endothelial cell line was donated by Professor Pierre-Olivier Couraud (Rnserm U567, Paris, France), the human glioblastoma cell lines U87 and U251 and the human embryonic kidney cell line HEK293 were purchased from the Life Science Cell Resource Center of Shanghai Research Institute . Vascular endothelial cells of glioma were cultured in EBM-2 medium specially for endothelial cells (add EBM-2 supplements according to the instructions, including VEGF, EGF, bFGF, hydrocortisone, ascorbic acid, gentamicin, fetal bovine serum) , replace the culture medium every 2 days, and the endothelial cells can grow to a single layer in about 5-7 days. Vascular endothelial cells of U87 glioma were cultured in DMEM high-glucose medium containing 10% fetal bovine serum, and the medium was changed every 2 days. The cells could grow to a monolayer in about 2-3 days. Va...

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Abstract

The invention belongs to the technical field of medicines and particularly relates to a targeting inhibitor for an MCM3AP-AS1 gene and an application of the targeting inhibitor. The targeting inhibitor has the gene sequence as follows: 5'-GCTGTACCTAGTATGGTATGC-3'(SEQ ID No.1). The inhibitor can be specifically bound with the MCM3AP-AS1 gene, so that the MCM3AP-AS1 gene is silenced, influences of MCM3AP-AS1 on vascular endothelial cell proliferation, migration and vessel formation capability of gliomas are inhibited, and the purpose of treating gliomas is achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a targeted inhibitor of MCM3AP-AS1 gene and its application. Background technique [0002] Glioma is the most common and most malignant central nervous system tumor in humans. Glioma is also a vascular-dependent solid tumor, when the tumor volume is greater than 2cm 2 , blood supply by new blood vessels becomes a necessary condition for tumor growth. Tumor vascular endothelial cells are stimulated in the glioma microenvironment to promote their proliferation and migration, and then form new blood vessels. Therefore, the biggest characteristics of gliomas are abundant new blood vessels, high invasiveness and poor prognosis. Although the comprehensive multimodal treatment of surgery, radiotherapy and chemotherapy has made great progress in recent years, due to the strong ability of vascular endothelial cells in glioma to proliferate, migrate and invade, after treatme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61K31/7088A61P35/00
CPCC12N15/1135C12N2310/113
Inventor 刘云会薛一雪蔡恒郑健刘啸白杨春清李振商秀丽
Owner SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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