Human bile duct cancer cell line, and applications thereof

A cancer cell and cholangiocarcinoma technology, applied in the field of cell lines, can solve the problems of not having Chinese genetic background, limited cholangiocarcinoma cell lines, and large differences in clinical bile duct cancer biological properties, achieving good uniformity and high scientific research Significance of value and development, effects of character stabilization

Active Publication Date: 2018-01-05
SHANGHAI EAST HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention...

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  • Human bile duct cancer cell line, and applications thereof
  • Human bile duct cancer cell line, and applications thereof
  • Human bile duct cancer cell line, and applications thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1LI

[0046] The preparation of embodiment 1LIXC-127 cell

[0047] A fresh resection specimen of clinical liver cancer (low to moderately differentiated adenocarcinoma of the intrahepatic bile duct, female, Han nationality, Chinese, 73 years old) was obtained from Shanghai Dongfang Hospital. Carcinoma with intrahepatic metastases.

[0048] Rinse the above specimen with fresh HBSS buffer (containing 500 U / mL penicillin G, 500 μg / mL streptomycin sulfate, and 1.25 μg / mL amphotericin B), cut into small pieces of 20-50 mg, subcutaneously puncture and inoculate in the immune system. Deficient animal SCID mice (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.); 70-90 days after puncture inoculation, the tumor-bearing animals were anesthetized and killed with excess carbon dioxide gas, and aseptically dissected. subculture and subculture.

[0049] Primary culture: cut the tumor tissue into small pieces, place them in culture flasks, and incubate at 37°C with 5...

Embodiment 2LI

[0052] Biological characteristics and application of embodiment 2LIXC-127 cells

[0053] The invention adopts RPMI1640 culture and purifies LIXC-127 cells by a differential attachment method, so that they can grow long-term in vitro and pass stably. When the cells are passed on for more than 20 generations, the cell traits are gradually stabilized, and relevant biology, genetics, and tissue source identification are carried out until the 30th to 50th generations have the same stable traits. According to experimental observation and verification, LIXC-127 grown in vitro has a typical epithelial-like morphology, the main body is flat irregular polygon, with a round nucleus in the middle, and grows in a covering layer. The cells adhere to the wall and are closely connected with each other. shape move. Genetic studies confirmed that the cells were aneuploid. The cells can form tumors in nude mice and have tumorigenicity. The corresponding relationship between the cells and the ...

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Abstract

The invention discloses a human bile duct cancer cell line, and applications thereof. The human bile duct cancer cell line is preserved at China Center for Type Culture Collection, and the preservation number is CCTCC NO:C201662. The main clinical biological characteristics of the human bile duct cancer cell line are maintainer, tumor formation rate is high, incubation period is short, and uniformity is high. The human bile duct cancer cell repository is enlarged, important research materials are provided for study based on genetic background of Chinese, and new experiment materials are provided for in vivo and in vitro experiments before new drug clinical study.

Description

technical field [0001] The invention belongs to the field of cell lines, in particular to a human cholangiocarcinoma cell line and application thereof. Background technique [0002] Cholangiocarcinoma is a kind of malignant tumor originating from epithelial cells, which can be divided into the following types according to its anatomical location: intrahepatic cholangiocarcinoma, hilar cholangiocarcinoma and extrahepatic cholangiocarcinoma. Although cholangiocarcinoma is uncommon, accounting for less than 2% of all cancers, its incidence is increasing worldwide. The incidence of cholangiocarcinoma ranks fifth among digestive tract malignant tumors in my country, and the age of onset is mostly between 50 and 70 years old, with slightly more men than women. About 2 / 3 of cholangiocarcinomas are located in the hilar part of the liver, that is, hilar cholangiocarcinoma, which includes the common hepatic duct, the confluence of the left and right hepatic ducts, and the cancer of t...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12Q1/02A61K49/00C12R1/91
Inventor 郑亚新过欣来杨冰谢付波李利林凯汪宗宇周禾
Owner SHANGHAI EAST HOSPITAL
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