Porcine kobuvirus quick detection method combining reverse transcription-recombinase polymerase amplification (RT-RPA) and lateral flow chromatographic technology, and porcine kobuvirus detection kit

Abstract

The invention discloses a specific primer and a probe for quickly detecting porcine kobuvirus by combining reverse transcription-recombinase polymerase amplification (RT-RPA) and a lateral flow chromatographic technology, and creates a porcine kobuvirus quick detection method combining RT-RPA and the lateral flow chromatographic technology. The method can detect 6.4*10<3>copies / mu L of porcine kobuvirus RNA at least, so that the sensitivity is much higher than that of an existing PCR detection method. The method can detect the porcine kobuvirus as early as 10 minutes later the reaction startsand can finish detection within 10-15 minutes. The specific primer and probe designed for quick detection of the porcine kobuvirus have strong specificity for porcine kobuvirus, the detection result can be seen intuitively by naked eyes, the detection process is convenient and quick, and the detection result is safe and reliable.

Description

technical field [0001] The invention belongs to the technical field of molecular biology detection of viruses, and in particular relates to a rapid detection method of porcine ridge virus and a detection kit thereof by combining RT-RPA and lateral flow chromatography technology. Background technique [0002] In recent years, nucleic acid isothermal amplification technology has developed rapidly. It gets rid of the traditional temperature cycler and can rapidly perform amplification reactions in a short time. In 2006, Piepenburg et al. first proposed recombinase polymerase amplification (RPA), which is a new nucleic acid isothermal amplification technology that simulates the DNA replication process in organisms. The amplification process can be performed in traditional reaction tubes. The target fragment can be amplified isothermally at 37°C-42°C, and the amplification reaction can be completed in 20 minutes. It has the technical advantages of rapid response and easy operatio...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems existing in the known technology, the present invention provides a rapid detection method of porcine crest virus and its detection kit by combining RT-RPA and lateral flow chromatography technology, which can quickly complete porcine crest virus at a lower temperature. The virus detection solves the problems of complex reaction procedures, low sensitivity, inconvenient carrying of detection instruments and inability to directly observe the detection results in the detection of porcine crest virus

Images