Preparation method of anti-aging whey protein peptide

A whey protein peptide, anti-aging technology, applied in the direction of fermentation, can solve the problems of rare, imperfect preparation method of whey protein peptide, etc.

Inactive Publication Date: 2018-01-12
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Whey protein peptide is a kind of low molecular weight peptide obtained by hydrolyzing whey protein in milk by enzymatic technology. It has been found that some peptides have anti-oxidation, lower blood pressure, lower cholesterol, enhance immunity, anti-

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  • Preparation method of anti-aging whey protein peptide
  • Preparation method of anti-aging whey protein peptide
  • Preparation method of anti-aging whey protein peptide

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Embodiment Construction

[0041] The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings, but are not limited thereto. Any modification or equivalent replacement of the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention shall be included in the present invention. within the scope of protection.

[0042] The screening of protease

[0043] The enzymatic activity was measured according to the conventional method for measuring the enzymatic activity, and the results are shown in Table 1.

[0044] Table 1 Substrate specificity of different proteases, the most suitable action conditions and their measured enzyme activities

[0045]

[0046] Then compare the hydrolysis effect of the five proteases, adjust the optimum pH and temperature of each enzyme, the enzyme dosage is 5000U / g, and the hydrolysis time is 3h. The degree of hydrolysis and DPPH f...

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Abstract

The invention discloses a preparation method of anti-aging whey protein peptide, belonging to the technical field of bioactive peptide. The preparation method comprises the following steps: hydrolyzing whey protein concentrate (WPC 80) by adopting alkaline protease, by adopting the degree of hydrolysis and the DPPH free radical scavenging rate as the indexes of evaluation, the hydrolysis and the DPPH free radical scavenging rate as the indexes, adjusting the influences of the following conditions on the hydrolysis effect: the enzyme dosage: 400-10000 U/g, the pH: 8.0, 8.5 and 9.0, the temperatures: 55 DEG C, 60 DEG C and 65 DEG C, the concentration of the substrate: 3-7 %, and the hydrolysis time: 1-6 h, thus the optimal hydrolysis process is obtained, and then enzymolysis is carried out according to the optimal process; hydrolyzing the whey protein concentrate (WPC 80) under the optimal process conditions, desalting the whey protein zymolyte by adopting DA201-C macroporous resin, carrying out dynamic desalting experiment under the following conditions: deionized water and 40-70 % alcohol are adopted as desorption reagents, the sample loading flow rate is 0.5-2.5 mL/min, and the sample loading concentration is 10-50 mg/mL, collecting peptide liquid obtained after desalting, carrying out ultra-filtration separation, and identifying the molecular weight of whey protein peptide byadopting Tricine-SDS-PAGE electrophoresis.

Description

technical field [0001] The invention relates to a preparation method of anti-aging whey protein, belonging to the technical field of biologically active peptides. Background technique [0002] With the development of modernization, population aging has become one of the issues that people are generally concerned about. With the growth of human beings, the metabolism of the body slows down, the cells gradually age, the organ function declines, and the aging of the brain is also increasing. The most obvious manifestations are the decline of learning and memory ability and cognitive function, and even induce the degeneration of the central nervous system. Diseases, such as Alzheimer's disease (AD), Parkinson's disease (PD), etc. The decline of the learning and memory ability of the elderly not only brings a lot of inconvenience to their personal life and seriously affects their quality of life, but also brings a heavy burden to the country, society and family. [0003] At pre...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16
Inventor 于国萍邵美丽岳崇慧卞辑刘鹏孙琪
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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