Method for identifying germplasm resource of easy-to-flower type Litchi chinensis Sonn.

A litchi and germplasm technology, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. The effect of wide application prospects

Inactive Publication Date: 2018-01-16
广西壮族自治区农业科学院园艺研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Litchi varieties that are difficult to grow into flowers (such as "Guiwei" and "Nuomici"), although the flowering is greatly affected by the lack of low temperature in winter, are favored by consumers due to their high quality and are gradually promoted on a large scale. The annual output cannot be guaranteed. For example, in 2017, due to the impact of insufficient low temperature in winter (warm winter), the output was reduced by about 80%.

Method used

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  • Method for identifying germplasm resource of easy-to-flower type Litchi chinensis Sonn.
  • Method for identifying germplasm resource of easy-to-flower type Litchi chinensis Sonn.
  • Method for identifying germplasm resource of easy-to-flower type Litchi chinensis Sonn.

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Embodiment 1

[0034] Example 1 : A method for identification of Yichenghua litchi germplasm resources

[0035] 1. Theoretical basis for the invention of a method for identifying the germplasm resources of Yichenghua litchi:

[0036] Low temperature can induce the expression of the key flowering gene LcFT1 in litchi leaves and lead to its flowering. There are two types of litchi LcFT1 gene promoters (namely "EFT" type and "DFT" type), which lead to three types of LcFT1 genotypes in litchi, respectively. It is homozygous "EFT" type litchi, homozygous "DFT" type litchi and the heterozygous litchi of the two, and the litchi containing the "EFT" type promoter is easy to flower, that is, the homozygous "EFT" type litchi and the heterozygous type litchi.

[0037] 2. A method for identifying Yichenghua litchi germplasm resources, comprising the steps of:

[0038] (1) Collect 72 different litchi germplasm resources (such as: "Sanyuehong", "Feizixiao", "Lanzhu", "Guizaoli", "Maguili", "Guiwei", "...

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Abstract

The invention belongs to the technical field of genetic breeding of Litchi chinensis Sonn., and particularly relates to a method for identifying a germplasm resource of easy-to-flower type Litchi chinensis Sonn.. The inventor develops two pairs of primers (FT-F and EFT-R, DFT-F and DFT-R) according to specificities of EFT type and DFT type promoters of LcFT1 gene to identify the germplasm resourceof the easy-to-flower type Litchi chinensis Sonn.. The method provided by the invention can identify the germplasm resource of the easy-to-flower type Litchi chinensis Sonn. in the DNA level, has thecharacteristics of being easy, having high efficiency, delivering accurate identification result, and being not affected by external environment, can help build a breeding system of new germplasm ofthe easy-to-flower type Litchi chinensis Sonn. and has a great application prospect.

Description

technical field [0001] The invention belongs to the technical field of litchi genetics and breeding, and in particular relates to a method for identifying germplasm resources of litchi which are prone to flowering. Background technique [0002] Litchi (Litchi chinensis Sonn.) is a Sapindaceae plant of the genus Litchi, which originated in China. It is known as the "Lingnan Fruit King" because of its fruit shape, color, aroma, taste and rich nutrition. Past production experience has shown that low temperature is the key external environmental factor required for flower induction in litchi, and it has also been found that a very small number of litchi varieties (such as "Sanyuehong" and "Brown Maoli") only need a short period of low temperature to complete flower formation. Flower induction, easy to flower, so it is called easy-to-flower litchi varieties; and most litchi varieties (such as "Guiwei", "Nuomici", "Ziliangxi") must go through the low temperature throughout the win...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 丁峰张树伟彭宏祥陈厚彬
Owner 广西壮族自治区农业科学院园艺研究所
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