Method for simply and rapidly detecting easy-to-blossom litchi germplasm resources

A lychee and resource technology is applied in the field of simple and rapid detection of litchi germplasm resources of Yichenghua, which can solve the problems of unguaranteed yield and yield reduction, and achieve the effects of easy automation, high separation efficiency, and clear selection goals.

Inactive Publication Date: 2018-01-16
广西壮族自治区农业科学院园艺研究所
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Problems solved by technology

Litchi varieties that are difficult to grow into flowers (such as "Guiwei" and "Nuomici"), although the flowering is greatly affected by the lack of low temperature in winter, are favored by consumers due to their high quality and are gradually promoted on a large scale. The annual output cannot be guaranteed. For example, in 2017, due to the impact of insufficient low temperature in winter (warm winter), the output was reduced by about 80%.

Method used

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  • Method for simply and rapidly detecting easy-to-blossom litchi germplasm resources
  • Method for simply and rapidly detecting easy-to-blossom litchi germplasm resources

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Embodiment 1

[0031] 1. The theoretical basis for the invention of a simple and rapid method for detecting litchi germplasm resources that are prone to flowering: low temperature can induce the expression of the flowering gene LcFT1 in litchi leaves and lead to flowering, such as figure 1 As shown, due to differences in promoter sequences (including small indels mutations), there are two types of litchi LcFT1 gene, low temperature-sensitive and low-temperature insensitive, and litchi containing the low-temperature-sensitive LcFT1 gene needs less low temperature for flower induction and is easy to grow. Flower, that is, the LcFT1 genotype of Yichenghua litchi germplasm resources is homozygous low temperature sensitive type or heterozygous low temperature sensitive type and low temperature insensitive type.

[0032] 2. A method for simple and rapid detection of litchi germplasm resources that are easy to grow flowers, comprising the steps of:

[0033] (1) Collect 72 different litchi germplasm...

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Abstract

The invention belongs to the technical field of genetic breeding of litchis and in particular relates to a method for simply and rapidly detecting easy-to-blossom litchi germplasm resources. The applicant develops a pair of primers LcCeED-F1 and LcCeED-R1 according to the difference between two types of LcFT1 genes and identifies the easy-to-blossom litchi germplasm resources based on a capillaryelectrophoresis technology. The method provided by the invention can be used for identifying the easy-to-blossom litchi germplasm resources based on a DNA (Deoxyribonucleic Acid) level; the method provided by the invention has the characteristics of simplicity, high efficiency, accurate identification result and the like and is not influenced by the external environment; the method has a wide application prospect in litchi breeding in the future.

Description

technical field [0001] The invention belongs to the technical field of litchi genetics and breeding, and in particular relates to a simple and rapid method for detecting germplasm resources of litchi which are easy to flower. Background technique [0002] Litchi (Litchi chinensis Sonn.) is a Sapindaceae plant of the genus Litchi, which originated in China. It is known as the "Lingnan Fruit King" because of its fruit shape, color, aroma, taste and rich nutrition. Past production experience has shown that low temperature is the key external environmental factor required for flower induction in litchi, and it has also been found that a very small number of litchi varieties (such as "Sanyuehong" and "Brown Maoli") only need a short period of low temperature to complete flower formation. Flower induction, easy to flower, so it is called easy-to-flower litchi varieties; and most litchi varieties (such as "Guiwei", "Nuomici", "Ziliangxi") must go through the low temperature through...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 张树伟丁峰彭宏祥陈厚彬
Owner 广西壮族自治区农业科学院园艺研究所
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