A kind of preparation method of bamboo leaf soluble dietary fiber
A dietary fiber and soluble technology, applied in food science, biofuel, fermentation, etc., can solve the problems of high energy consumption, environmental pollution by chemical methods, low yield, etc., to prevent and treat gallstones, reduce human serum cholesterol, and enrich soluble meals fiber effect
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Embodiment 1
[0026] Embodiment 1: the bamboo leaves after picking are washed with running water, dried, ground and crossed a 60-mesh sieve for subsequent use. Weigh 50g of bamboo leaf powder with hot water at a material-to-liquid ratio of 1:20g / ml, extract in a water bath at 80°C for 30 minutes, and extract twice to remove water-soluble substances such as soluble sugars and amino acids; filter, and filter residues in an oven Dry at 70°C for later use. Weigh 10 g of filter residue, dissolve in 200 mL of citrate buffer solution with pH 6.0, add 0.2 g of α-amylase, and perform enzymatic hydrolysis at 40° C. for 4 hours. Adjust the pH of the enzymolysis solution to 4.2, add 0.003g CaCl 2 and 0.6g cellulase at 45°C for 1 hour. Adjust the pH of the enzymolysis solution to 6.6, add 0.6 g of papain and perform enzymolysis at 55° C. for 1 hour. Boil the enzymolysis solution for 10 minutes to inactivate the enzyme, centrifuge, and separate the supernatant from the precipitate. Wash the pellet th...
Embodiment 2
[0027] Embodiment 2: the bamboo leaves after plucking are washed with running water, dried, and ground through an 80-mesh sieve for subsequent use. Weigh 200g of bamboo leaf powder with hot water at a material-to-liquid ratio of 1:15g / ml, leaching in a 90°C water bath for 60 minutes, and leaching 3 times to remove soluble sugars, amino acids and other water-soluble substances; filter, and filter residues in an oven Dry at 90°C for later use. Weigh 100 g of filter residue, dissolve in 400 mL of citrate buffer solution with pH 6.8, add 6 g of α-amylase, and perform enzymatic hydrolysis at 50° C. for 6 hours. Adjust the pH of the enzymolysis solution to 5.0, add 0.1g CaCl 2 and 10 g of cellulase at 55° C. for 3 hours. Adjust the pH of the enzymolysis solution to 7.0, add 12 g of papain and perform enzymolysis at 60° C. for 3 hours. Boil the enzymolysis solution for 20 minutes to inactivate the enzyme, centrifuge, and separate the supernatant from the precipitate. Wash the pel...
Embodiment 3
[0028] Embodiment 3: the bamboo leaves after plucking are washed with running water, dried, and ground through a 60-mesh sieve for subsequent use. Weigh 100g of bamboo leaf powder with hot water at a material-to-liquid ratio of 1:18g / ml, extract in a water bath at 85°C for 45 minutes, and extract 3 times to remove water-soluble substances such as soluble sugars and amino acids; filter, and filter residues in an oven Dry at 80°C for later use. Weigh 30 g of filter residue, dissolve in 200 mL of citrate buffer solution with pH 6.0, add 1.2 g of α-amylase, and perform enzymatic hydrolysis at 45°C for 5 hours. Adjust the pH of the enzymolysis solution to 4.6, add 0.0192g CaCl 2 and 2.4g cellulase at 50°C for 2 hours. Adjust the pH of the enzymolysis solution to 6.8, add 2.7 g of papain and perform enzymolysis at 58° C. for 2 hours. Boil the enzymolysis solution for 15 minutes to inactivate the enzyme, centrifuge, and separate the supernatant from the precipitate. Wash the pell...
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