Method for detecting clenbuterol in swine urine on basis of Fourier transformation infrared spectrum technology

A technology based on Fourier transform and infrared spectroscopy, which is applied in the field of detection of clenbuterol in pig urine based on Fourier transform infrared spectroscopy, can solve the problem of "abuse and circulation of clenbuterol, high cost of reagent consumables, sampling Detection and other problems, to achieve the effect of eliminating interference to infrared spectrum, maintaining health benefits, and easy operation

Inactive Publication Date: 2018-02-09
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection methods for ractopamine, clenbuterol, and albuterol mainly include colloidal gold test strip method, gas chromatography-mass spectrometry, high-performance liquid chromatography-mass spectrometry, enzyme-linked immunosorbent assay, etc. Most of these detection methods have High cost, large false positive ratio, sampling test, environmental pollution and other defects make it difficult to effectively curb the abuse and circulation of "Clenbuterol". It still needs to be stored at low temperature and has a certain shelf life, and is easily interfered by other "lean meat powder" components and chemical substances. The used and expired test strips are not centrally disposed of. The annual consumption cost of test strips for large and medium-sized slaughtering enterprises is at least 6 million yuan; ②Gas chromatography-mass spectrometry, high performance liquid chromatography-mass spectrometry and enzyme-linked immunosorbent assay: all of these methods need to concentrate the samples, so they need to consume a lot of reagent consumables, showing high cost and certain environmental pollution , it also requires professional operators and a complete special laboratory, it is difficult to detect in batches, and the detection cycle is long, about 10 hours / sample

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  • Method for detecting clenbuterol in swine urine on basis of Fourier transformation infrared spectrum technology
  • Method for detecting clenbuterol in swine urine on basis of Fourier transformation infrared spectrum technology

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Experimental program
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Embodiment 1

[0025] When ractopamine, clenbuterol, and albuterol in pig urine exist alone, the specific steps for detecting their content using Fourier transform infrared spectroscopy are as follows:

[0026] (1) Sample pretreatment: collect pig urine, let it stand for 30 minutes, take 3ml of supernatant and dry it for 40 hours at a temperature of 40°C and a wind speed of 2m / s to obtain viscous urine;

[0027] (2) Preparation of sample sheet: Take 100 mg of dry potassium bromide powder with a particle size of 200 mesh, put it into a mold and press it into a potassium bromide wafer, and then apply the viscous urine obtained in step (1) evenly on the surface of the potassium bromide wafer to obtain a sample to be tested sample sheet;

[0028] (3) Spectrum acquisition: using Fourier transform infrared spectrometer, set the resolution to 8cm -1 , The spectral range is 4000~400cm -1 , the apodization function is Happ-Genzel, with the potassium bromide wafer as the background, the accumulative...

Embodiment 2

[0036] As the three commonly used "lean meat extracts" ractopamine, clenbuterol and albuterol, if any two of them are mixed in pig urine, the specific operation steps for detecting their content by Fourier transform infrared spectroscopy are as follows:

[0037] (1) Sample pretreatment: collect pig urine, centrifuge at 1500×g for 5 minutes, take 3ml of supernatant and dry it for 36 hours at a temperature of 38°C and a wind speed of 3m / s to obtain viscous urine;

[0038] (2) Preparation of sample sheet: Take 100 mg of dry potassium bromide powder with a particle size of 200 mesh, put it into a mold and press it into a potassium bromide wafer, and then apply the viscous urine obtained in step (1) evenly on the surface of the potassium bromide wafer to obtain a sample to be tested sample sheet;

[0039] (3) Spectrum acquisition: using Fourier transform infrared spectrometer, set the resolution to 8cm -1 , The spectral range is 4000~400cm -1 , the apodization function is Happ-Ge...

Embodiment 3

[0047] When ractopamine, clenbuterol and albuterol are mixed in pig urine, the method for simultaneously detecting the contents of these three "leanbuterol" using Fourier transform infrared spectroscopy, the specific operation steps are as follows:

[0048] (1) Sample pretreatment: collect pig urine, let it stand for 30 minutes, take 3ml of supernatant and dry it for 48 hours at a temperature of 35°C and a wind speed of 1m / s to obtain viscous urine;

[0049] (2) Preparation of sample sheet: Take 100 mg of dry potassium bromide powder with a particle size of 200 mesh, put it into a mold and press it into a potassium bromide wafer, and then apply the viscous urine obtained in step (1) evenly on the surface of the potassium bromide wafer to obtain a sample to be tested sample sheet;

[0050] (3) Spectrum acquisition: using Fourier transform infrared spectrometer, set the resolution to 8cm -1 , The spectral range is 4000~400cm -1 , the apodization function is Happ-Genzel, with t...

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Abstract

The invention discloses a method for detecting clenbuterol in swine urine on the basis of a Fourier transformation infrared spectrum technology. The method comprises the following steps: acquiring spectral information of pretreated urine by using Fourier transformation infrared spectrum equipment, and quickly realizing quantitative detection of clenbuterol in the swine urine by using a built detection model after data processing and analysis modeling. The method has the advantages of simplicity and convenience in operation, low cost and no environmental pollution, can effectively avoid defectscaused by conventional analysis, is feasible to application to batch detection in the slaughtering industry, and can guarantee the health benefits of customers.

Description

technical field [0001] The invention relates to the technical field of animal excrement detection, in particular to a method for detecting clenbuterol in pig urine based on Fourier transform infrared spectroscopy. Background technique [0002] Ractopamine, clenbuterol and salbutamol are often used as illegal drugs to suppress fat and promote lean growth, commonly known as "clenbuterol". Such drugs have the characteristics of slow metabolism and easy enrichment in animals, and are easily ingested by the human body through the food chain, causing a series of clinical poisoning symptoms such as nausea, palpitations, facial tightness, and rapid heartbeat, which can be life-threatening in severe cases. An important hazard factor for the safety of meat products. In view of the fact that such drugs are mainly excreted in urine in animals, the industry can reduce the safety risk of meat and meat products after slaughter by monitoring the relevant drug residues in their urine. [0...

Claims

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Application Information

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IPC IPC(8): G01N21/3577G01N1/28
CPCG01N1/286G01N21/3577G01N2021/3595
Inventor马飞王学瑞周颖陈从贵郑磊牛向丽王纯贤张永亮
OwnerHEFEI UNIV OF TECH