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Chromatographic analysis device and chromatographic analysis method

A chromatographic analysis and chromatographic medium technology, applied in the direction of measuring devices, analytical materials, biological material analysis, etc., can solve the problems of sensitivity improvement, achieve the effect of preventing adverse effects and improving detection sensitivity

Active Publication Date: 2018-02-16
TANAKA PRECIOUS METAL IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, in conventional immunochromatographic assay devices, it is impossible to suppress non-specific reactions while increasing sensitivity to the desired level

Method used

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  • Chromatographic analysis device and chromatographic analysis method
  • Chromatographic analysis device and chromatographic analysis method
  • Chromatographic analysis device and chromatographic analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0102] Hereinafter, the present invention will be further described through examples, but the present invention is not limited to the following examples.

[0103] (1) Preparation of the sample addition part

[0104] A nonwoven fabric (manufactured by Millipore Corporation: 300 mm×30 mm) made of glass fibers was used as the sample adding portion.

[0105] (2) Preparation of marker holding part

[0106] 0.1 mL of mouse-derived anti-RS virus monoclonal antibody diluted to a concentration of 0.05 mg / mL with phosphate buffer (pH 7.4) was added to 0.5 mL of gold colloidal suspension (manufactured by Tanaka Kikinzoku Kogyo Co., Ltd.: LC40nm), and let stand at room temperature for 10 minutes.

[0107] Next, 0.1 mL of a phosphate buffer solution (pH 7.4) containing 1% by mass of bovine serum albumin (BSA) was added, and the mixture was further left to stand at room temperature for 10 minutes. Thereafter, after sufficiently stirring, centrifugation was performed at 8000×g for 15 minu...

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Abstract

The present invention provides a chromatographic analysis device capable of improving the detection sensitivity of a substance to be detected while inhibiting a nonspecific reaction. The present invention relates to a chromatographic analysis device which includes at least a chromatograph medium section (3) supported by a detection part (4) for detecting a substance to be detected contained in a sample. The chromatograph medium section (3) has a configuration in which a membrane is disposed on a support. The average thickness of the membrane is 110 to 130 mum, and the development current velocity of the membrane is 30 to 45 seconds / 40 mm.

Description

technical field [0001] The invention relates to a chromatographic analysis device and a chromatographic analysis method. Background technique [0002] In recent years, in immunoassays using immunochromatography that do not require pretreatment of specimens, they have been used as simple in vitro diagnostic kits or portable diagnostic kits for detecting antigens in sample liquids using the specific reactivity of antibodies. device, its importance is increasing. In particular, pathogen test kits such as viruses and bacteria are personal immunochromatographic analyzers that are widely used in general hospitals and clinics. [0003] As the simplest structure of a conventional immunochromatographic analysis device, a sample adding part, a labeling substance holding part, a chromatography medium part carrying a detecting part, and an absorbing part that absorbs the liquid passing through the detecting part are connected to each other. Structure. [0004] Such an immunochromatog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/54393G01N33/54388G01N33/54387G01N33/56983G01N2333/135
Inventor 铃木启太岩本久彦
Owner TANAKA PRECIOUS METAL IND