Tissue culture rapid propagation culture medium of cymbidium floribundum

Abstract

The invention provides a tissue culture rapid propagation culture medium of cymbidium floribundum, belonging to the technical field of plant propagation. The tissue culture rapid propagation culture medium comprises an induced culture medium, a subculture medium and a strong seedling culture medium; and moringa oleifera leaf juice and lycium barbarum leaf juice are added in the culture mediums used in all culture stages to be taken nutritional agents. A formula of the induced culture medium is as follows: 3g / L of Hyponex No.1, 1g / L of active carbon, 1mg / L-2mg / L of gibberellins, 5.0g / L-8.0g / L of agar, 2.0g / L-4.0g / L of saccharose, 5.0g / L-8.0g / L of moringa oleifera leaf juice and 3.0g / L-5.0g / L of lycium barbarum leaf juice, and the pH value is 5.4-5.6. By adoption of the formula of the culture medium provided by the invention, the induced differentiation rate, the multiplication coefficient of the explants and the rooting rate of the cymbidium floribundum can be obviously increased, the culture period can be shortened and the quality of the tissue culture seedlings of the cymbidium floribundum can be improved.

Description

【Technical field】 [0001] The invention relates to the technical field of plant propagation, in particular to a culture medium for tissue culture and rapid propagation of orchid multiflora. 【Background technique】 [0002] Cymbidium floribundum (Cymbidium floribundum) is an epiphytic plant of the genus Orchid, which belongs to Appendix II species of the Convention on International Trade in Endangered Species of Wild Fauna and Flora, and is a second-class protected plant in my country. Duohualan is mainly distributed in Yunnan, Guangxi, Guangdong, Fujian and other provinces south of the Yangtze River in my country; it grows in forests at an altitude of 100-3300m, on forest edge trees, or on rock walls along valleys. Its leaves are leathery, shiny, and strong in drought and cold resistance; the flowers are fragrant, have a long flowering period, colorful flowers, and have high ornamental value. It is one of the more ideal potted flowers. [0003] Multi-flowered orchids are easy...

AI Technical Summary

Problems solved by technology

At present, there are also some scholars who have studied the method of obtaining Orchid multiflora seedlings by using Orchid multiflora capsules or stem tips as explants for tissue culture, but due to the lack of related research, they have not found a method that is more suitable for the growth of shoot tip differentiation tissues. In the process of tissue culture, the culture period is long and the culture effect is unsatisfactory