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Composite nano-particle for three-dimensional fluorescence imaging of blood vessel network, and preparation method and application thereof

A composite nanoparticle and network three-dimensional technology, which can be used in preparations, chemical instruments and methods, and luminescent materials for in vivo experiments. Luminescent performance, excellent high stability, simple and easy preparation method

Active Publication Date: 2018-02-23
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of poor fluorescence stability, easy leakage of fluorescent materials, high price, and complicated preparation methods in the prior art, the inventors conducted research and proposed a new type of composite nanoparticle
The composite nanoparticles designed in the present invention can be uniformly dispersed and fixed in the blood vessels. When used for fluorescence imaging, the brightness is high, the dyeing is uniform, the cost is low, and multi-channel imaging can be realized. It can achieve high-resolution three-dimensional imaging of the fine network structure of blood vessels due to many problems such as uniformity, easy blockage of blood vessels, and high cost.

Method used

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  • Composite nano-particle for three-dimensional fluorescence imaging of blood vessel network, and preparation method and application thereof
  • Composite nano-particle for three-dimensional fluorescence imaging of blood vessel network, and preparation method and application thereof
  • Composite nano-particle for three-dimensional fluorescence imaging of blood vessel network, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Preparation of surface amino-modified polystyrene nanoparticles (taking polystyrene nanoparticles with an average particle size of 150 nm as an example)

[0031] (1), 0.025g of sodium lauryl sulfate and 0.2g of sodium bicarbonate were dissolved in 250ml of ultrapure water, heated under vigorous stirring to make them completely dissolved, and 15g of polystyrene was added, and continued at room temperature Stir, and add a saturated solution containing 10 g of potassium persulfate to the system. Under the protection of argon atmosphere, the reaction was carried out at 70° C. for 10 h. After the reaction, the supernatant was removed by centrifugation, washed three times with ultrapure water and absolute ethanol, dried and stored at room temperature.

[0032] (2) Weigh 1.0 g of the material prepared in the above step (1), disperse it evenly in ultrapure water, and add 25 g of concentrated HNO dropwise to the system under stirring 3 / Concentrated H2 SO 4 Mix the soluti...

Embodiment 2

[0044] 1. Preparation of surface amino-modified polystyrene nanoparticles (taking polystyrene nanoparticles with an average particle size of 150 nm as an example)

[0045] (1), 0.025g of sodium lauryl sulfate and 0.2g of sodium bicarbonate were dissolved in 250ml of ultra-pure water, heated under vigorous stirring to make them completely dissolved, and 12.5g of polystyrene added was continued at room temperature Under stirring, add a saturated solution containing 10 g of potassium persulfate to the system. Under the protection of argon atmosphere, the reaction was carried out at 70° C. for 10 h. After the reaction, the supernatant was removed by centrifugation, washed three times with ultrapure water and absolute ethanol, dried and stored at room temperature.

[0046] (2) Weigh 1.0 g of the material prepared in the above step (1), disperse it evenly in ultrapure water, and add 25 g of concentrated HNO dropwise to the system under stirring 3 / Concentrated H 2 SO 4 Mix the ...

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Abstract

The invention discloses a composite nano-particle for three-dimensional fluorescence imaging of a blood vessel network, and a preparation method thereof and an application thereof. The composite nano-particle includes 1-30 wt% of a fluorescent material used as a core and 70-99 wt% of an amino-modified polymer used as a shell layer. The fluorescent material has the advantages of controllable dimensions, good water dispersibility, high light stability, and simple and easy preparation process. The prepared composite nano-particle has the advantages of high fluorescence brightness, uniformity in dyeing, low interference of photobleaching and background fluorescence, low toxicity and low cost when used for the blood vessel imaging, can accurately mark the fine structure of the blood vessel network, and has potential practical values in in-vivo imaging, medical diagnosis, disease therapy and other important fields.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a composite nanoparticle for three-dimensional fluorescence imaging of a blood vessel network, a preparation method and an application thereof. Background technique [0002] In recent years, fluorescence imaging technology has received high attention in the field of biomedicine as an efficient visualization research method, and has shown broad application prospects in aspects such as gene expression, biological information transmission, and real-time monitoring of living physiological processes. Compared with traditional imaging techniques, fluorescence imaging techniques have significant advantages such as high sensitivity, less biological damage, high imaging resolution, and real-time monitoring. [0003] Vascular imaging using fluorescence technology is of great significance in biological science research and disease diagnosis. The three-dimensional reconstruction of the fine netwo...

Claims

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Application Information

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IPC IPC(8): C09K11/02A61K49/00
CPCA61K49/0017A61K49/0093C09K11/025
Inventor 张浩力曹晶晶龚晓婷张胜祥成晓峰谢文广
Owner LANZHOU UNIVERSITY
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