A SERS-enhanced controllable chain-like assembly for detection of thrombin

A technology of thrombin and assembly, applied in measuring devices, material stimulation analysis, instruments, etc., can solve the problems of antibody stability being easily affected by the environment, long antibody preparation cycle, and affecting the sensitivity and specificity of detection results, etc., to achieve Strong Raman signal enhancement effect, quantitative detection, and strong Raman enhancement effect
CN107727632BActive Publication Date: 2020-08-04嘉兴千纯生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
嘉兴千纯生物科技有限公司
Publication Date
2020-08-04

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Abstract

The invention relates to a method for detecting thrombin by virtue of an SERS enhanced controllable chain-shaped assembly, belonging to the field of nano-sensing detection. According to the method, DNA molecules of two aptamers of the thrombin are respectively modified to the surfaces of an Au@ nano-particle and an Ag nano-particle, an alternate-arrayed chain-shaped structure is formed between thetwo nano-particles by controlling the DNA modification amounts of the surfaces of the nano-particles through the combination between assembly molecules and the thrombin when the thrombin exists, anda relatively strong Raman signal enhancement effect is achieved; and when the content of the thrombin is higher, the chain structure is longer, and the Raman signal strength is stronger, and the quantitative determination of the thrombin can be realized according to the corresponding relation between the content of the thrombin and the Raman signal strength. The method is simple in operation and high in sensitivity, and the thrombin in a serum sample can be detected; and by utilizing the nano-structure assembly, an important theoretical foundation is provided for detection of other target objects taking the aptamers as recognition molecules.
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Description

technical field

[0001] The invention belongs to the field of nanometer sensing and detection, and in particular relates to a method for detecting thrombin with a controllable chain assembly enhanced by SERS. Background technique

[0002] Protein is the main carrier of life activities and the main executor of biological functions. Almost all physiological and pathological processes can cause corresponding changes in proteins. Therefore, protein detection is widely used in basic life science research, human health evaluation, disease diagnosis, medicine and food. It has high application value in analysis and biosafety monitoring, and the detection of disease-related proteins in the field of biomedical diagnosis is a very important task.

[0003] At present, the commonly used methods for protein detection are immunoassay methods based on the specific recognition of antigens and antibodies, mainly including Western Blot, enzyme-linked immunosorbent adsorption (ELISA), affinity c...

Claims

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