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Culture media for promoting growth of Cypripedium palangshanense

A culture medium and indole butyric acid technology, applied in the field of plant tissue culture, can solve the problems of scarcity and extinction, achieve low cost, ensure balanced nutrition, and be suitable for popularization and application

Inactive Publication Date: 2018-03-20
陶象余
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the latest discoveries in recent years, Balangshan Cypedium is a medicinal plant with high medicinal value, but due to its special growth environment and conditions, its number is scarce and it is facing the crisis of extinction. Therefore, the development of It is very necessary to develop a medium for Cypripedium balangshan for artificial cultivation

Method used

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  • Culture media for promoting growth of Cypripedium palangshanense

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Experimental program
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Effect test

Embodiment 1

[0014] A culture medium for promoting the growth of Balangshan Cypripedium, characterized in that it comprises the following components:

[0015] 1 / 4MS medium, 0.2mg / L indolebutyric acid, 1mg / L gibberellin, 0.8mg / L triacontanol, 0.02mg / L atrazine, 0.3mg / L indole ester, 5g / L agarose.

Embodiment 2

[0017] A culture medium for promoting the growth of Balangshan Cypripedium, characterized in that it comprises the following components:

[0018] 1 / 2MS medium, 0.4mg / L indolebutyric acid, 0.8mg / L gibberellin, 1.2mg / L triacontanol, 0.07mg / L atrazine, 0.8mg / L indole ester, 3g / L L agarose.

Embodiment 3

[0020] A culture medium for promoting the growth of Balangshan Cypripedium, characterized in that it comprises the following components:

[0021] 1 / 4MS medium, 0.3mg / L indolebutyric acid, 0.9mg / L gibberellin, 1mg / L triacontanol, 0.02mg / L atrazine, 0.3mg / L indole ester, 4g / L agarose.

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Abstract

The invention discloses a culture media for promoting growth of Cypripedium palangshanense comprising following components: 1 / 4-1 / 2MS medium, 0.2-0.4mg / L indole butyric acid, 0.8-1mg / L gibberellin, 0.8-1.2mg / L triacontanol, 0 .02-0.07mg / L atrazine, 0.3-0.8mg / L ethychlozate and 3-5g / L agarose. The MS medium in the invention has less added components, significant culture effect and low cost; the addition of indole butyric acid, gibberellin and triacontanol can effectively promote the growth of roots and stems and leaves; the addition of atrazine and ethychlozate can further supplement the proteins and amino acids needed for cultivation to ensure balanced nutrition and increase survival rate to over 90%; the culture media is suitable for promotion and application.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a culture medium for promoting the growth of Balangshan Cypripedium. Background technique [0002] Cypripedium palangshanense Tang&F.T.Wang (scientific name: Cypripedium palangshanense Tang&F.T.Wang) is produced from the west to the southwest of Sichuan, my country, and is born in the forest or bush at an altitude of 2200-2700 meters. Its plants are 8-13 cm tall, with slender and transverse rhizomes. Stems erect, glabrous, mostly enclosed in several sheaths, with 2 leaves at the top. Leaves opposite or nearly opposite, flat; blades nearly round or nearly broadly elliptic. Inflorescence terminal, suberect, with 1 flower; flowers drooping, blood red or lavender; petals obliquely lanceolate, 1.2-1.6 cm long, 4-5 mm wide, apex acuminate, back base slightly hairy; Lip sac-shaped, subspherical, about 1 cm long, with a broad, nearly round sac opening; staminodes ovate-lanceolate, ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G24/00A01G24/30A01G24/10C05G3/00
CPCC05B7/00C05G3/00C05G5/23C05C1/00C05C5/02C05C11/00C05D1/00C05D3/00C05D5/00C05D9/00C05D9/02C05F11/00C05F11/10
Inventor 陶象余
Owner 陶象余