A Molecular Identification Method of Coptis Rhizoma

Abstract

The invention discloses a standard gene database for molecular identification of Coptis chinensis. The nucleotide sequence is the sequence in the sequence table, including the ycf1 sequence matrix of Coptis chinensis plants, including 19 species of Coptis chinensis plants known in the world. The molecular identification method Including: 1: extraction of the total DNA of the sample; 2: PCR amplification of the ycf1 gene fragment; 3: splicing the ycf1 sequence sequencing results to obtain the ycf1 gene fragment; 4: constructing the NJ tree of the ycf1 sequence of the sample; The advantages of the invention: a specific primer pair suitable for ycf1 sequence amplification of Coptis chinensis is designed, which can efficiently obtain the target sequence; no matter whether the fake Coptis chinensis comes from domestic or foreign countries, it can be accurately identified; the traditional method is used in Coptis chinensis The identification efficiency of the ycf1 sequence is only 20%-40%, while the identification efficiency of the ycf1 sequence of the present invention is more than 95%, which greatly improves the success rate of molecular identification of the traditional Chinese medicine Coptis chinensis.

Description

technical field [0001] The invention relates to molecular identification technology of Chinese medicinal materials, in particular to a method for quickly identifying Chinese medicinal Coptis chinensis medicinal materials stipulated in Pharmacopoeia by using self-designed specific primers to amplify chloroplast gene ycf1 fragments and its application. Background technique [0002] Coptis chinensis is one of the most well-known and commonly used traditional Chinese medicines in my country. It was first recorded in "Shen Nong's Materia Medica" and was listed as a top grade. "Pharmacopoeia of the People's Republic of China" (2010, part one) listed C. deltoidea C.Y. Cheng & P.K. Hsiao, C. teeta Wallich and C. teeta Wallich from the genus Coptis Salisb. Coptis chinensis (C. chinensis Franch.) is recorded as the original plant of Chinese medicine Coptis chinensis. As we all know, the accurate identification of Chinese medicinal materials is the premise and guarantee for studying t...

AI Technical Summary

Problems solved by technology

In 2012, Liu Xiaoguang first applied the above-mentioned standard barcode fragments to the molecular identification research of Coptis chinensis, but found that these DNA fragments had limited variation in Coptis genus plants, and the identification ability was only 20%-40%, which could not accurately identify the Chinese medicine Coptis chinensis. It shows that the plant standard DNA barcodes rbcL, matK, psbA-trnH and ITS recommended by the plant working group of the International Barcode Consortium of Life cannot be effectively used in the molecular identification of Chinese medicine Coptis chinensis. is an urgent issue

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