Anti-transferrin-containing recombination and attenuation salmonella enteritidis, construction method, expression or delivery carrier

Abstract

The invention belongs to the field of a bioengineering technology, and discloses an anti-transferrin-containing recombination and attenuation salmonella enteritidis, a construction method, expressionor a delivery carrier. The salmonella enteritidis is cultured by an LB culture medium, and a competent bacterium is prepared; a plasmid is electro-transformed into SL7207, a positive clone is acquiredin a culture medium containing Zeocin resistance, and corresponding rSL7207 is prepared; an RAW264.7 cell is infected by the rSL7207, and the expression of anti-transferrin in a macrophage is detected. An experimental result shows that the rSL7207 effectively kills BCG (Bacillus Calmette Guerin) infected with the macrophage, and provides a solid experimental foundation for a follow-up therapeuticanti-tuberculosis vaccine; the recombination and attenuation salmonella enteritidis rSL7207 is infected with the macrophage so as to successfully express the anti-transferrin; the rSL7207 can effectively kill the BCG infected with the macrophage. A mouse BCG infection model is built later so as to be used for more comprehensively evaluating a function of the rSL7207 in treating tuberculosis infection.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a recombinant attenuated Salmonella containing ferristatin, a construction method, and an expression or delivery vector. Background technique [0002] Tuberculosis is a disease that seriously threatens human health, and the infected people account for about one-third of the world's total population. According to the latest WHO global tuberculosis control report, there were about 9 million new cases and 1.5 million deaths in 2016, the number of deaths ranked first among single infectious diseases, and the number of tuberculosis patients in my country ranked second in the world. At present, there are several major problems in the prevention and control of tuberculosis: ①A large number of latent infections: Mycobacterium tuberculosis (Mtb) is an intracellular parasite, and the infection mainly parasitizes in macrophages (MΦ) and escapes immune killing through its ...

AI Technical Summary

Problems solved by technology

[0003] In summary, the problems in the prior art are: there are a large number of latent infections in the current tuberculosis prevention and treatment methods, the prevalence of multi-resistant strains (MDR), the co-infection of immunocompromised populations; latent infections, when there is no disease, tuberculosis The bacilli are latent in the macrophages in the human body, and the current anti-tuberculosis drugs are ineffective at this time

The prevalence of resistant strains (MDR) is not effective with current antibiotics

Images