A hybridization capture kit and method for detection of 16 gene hotspots in individualized medicine for tumors

Abstract

A hybridization capture kit for detection of 16 gene hotspots in tumor individualized medicine disclosed by the present invention includes hybridization reagents, PCR amplification reagents and enrichment detection reagents; wherein the hybridization reagents include the following SEQ NO. 1‑SEQ NO.396 probe mixture, and the molar ratio between each probe is 1:1. When detecting, the amount of the above probe mixture with a concentration of 10nM each is 1μl. The kit of the present invention is a gene detection based on next-generation sequencing technology, which can detect three types of mutations (mutation, insertion-deletion, fusion) at one time, and at the same time apply high sequencing depth to cover trace gene variations, so that it can be accurate under the premise of limited samples. Detect the mutation status of patients, and can accurately detect the mutation frequency of 1%. On the one hand, the high-sensitivity parallel detection of multiple genes improves the detection rate of gene mutations that can be used for drugs, so that the opportunity for medication is not missed; on the other hand, hotspots, rare or even unknown gene mutations can be detected simultaneously in one detection, While discovering drug-sensitive mutations, detect related drug-resistant mutations (such as KRAS and ALK point mutations), which accurately reflect the sensitivity and drug-resistance information of patients after medication.

Description

technical field [0001] The invention relates to the field of gene mutation detection, in particular to a hybrid capture kit and a detection method for detecting 16 gene hotspots in individualized medicine for tumors. Background technique [0002] With the discovery of tumor driver genes and the research and application of corresponding targeted drugs, tumor therapy has embarked on the road of individualized gene-oriented therapy. Targeted therapy has high efficiency and low side effects, which can improve the quality of life of lung cancer patients to a greater extent. However, targeted therapy drugs only act on tumor cells with specific gene mutations. For tumor cells without specific gene mutations, targeted drugs are even less effective than chemotherapy drugs. Therefore, patients must undergo corresponding genetic testing before receiving targeted therapy. [0003] The DNA derived from tumor tissue carries the unique gene characteristics of tumor cells, including gene ...

AI Technical Summary

Problems solved by technology

Among them, hybrid capture is characterized by the ability to capture exome or even larger target regions, with high coverage, high uniformity, high sequencing depth and high sensitivity, and can match extremely short template fragments or DNA with special structures , but the operation process is more complicated than multiplex PCR capture technology, and sometimes needs to rely on more specialized equipment; multiplex PCR is simple and flexible in operation, the template requirement is relatively low, the requirements for instruments are minimized, and the goal can be completed within a few hours Sequence enrichment and library construction are generally only suitable for capturing relatively small target sequences below 50Kb, and cannot detect gene rearrangements such as gene fusions or inversions, and large fragment deletions

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