Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting and preparing anthocyanins by tea callus suspension culture

A technology of callus and suspension culture, which is applied in botany equipment and methods, horticultural methods, chemical instruments and methods, etc., can solve the problem of high anthocyanin production costs, hindering the development of anthocyanin products, and failing to meet market demand And other issues

Active Publication Date: 2020-10-16
HUNAN AGRICULTURAL UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although suspension culture technology has been applied in several plants and has improved the efficiency of anthocyanin production to some extent, it has not been able to meet the market demand
[0005] In summary, the market prospect of anthocyanins is very broad, but the current production cost of anthocyanins is too high, which hinders the further development of anthocyanins products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting and preparing anthocyanins by tea callus suspension culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Select the vigorously growing "Jianbohuang" tea tree, take the third expanded leaf from each new shoot on the same tea tree, a total of 5 leaves, soak in 1% volume concentration of sodium hypochlorite aqueous solution for 18 minutes, take it out, and rinse with sterilized water Sodium hypochlorite solution, dried with sterile wind, under aseptic conditions, the leaves were cut into small pieces with an area of ​​0.8-1.2 square centimeters; the small pieces of leaves were transferred to callus culture medium (2.2g / L MS salt+0.05mg / L TDZ+0.05mg / L IBA+0.10mg / L GA 3 +20.0g / L sucrose+6g / L agar powder), put 10 small pieces of tea leaves in each petri dish, and cultivate 10 petri dishes in total. The culture conditions are: 21°C, 10 hours of light, 14 hours of darkness, light The strength is 1200lux, the relative humidity is 70%, and the new callus medium with the same composition is transferred to subculture every 2 weeks; the red callus begins to appear in the fourth week, ...

Embodiment 2

[0023]Select the vigorously growing "Jianbohuang" tea tree, take the third expanded leaf from each new shoot on the same tea tree, a total of 5 leaves, soak in 1% sodium hypochlorite aqueous solution for 20 minutes, take it out, and rinse it with sterilized water Sodium hypochlorite solution, dried with sterile wind, under aseptic conditions, the leaves were cut into small pieces with an area of ​​0.8-1.2 square centimeters; the small pieces of leaves were transferred to callus culture medium (2.2g / L MS salt+0.2mg / L TDZ+0.10mg / L IBA+0.20mg / L GA 3 +20.0g / L sucrose+6g / L agar powder), put 10 small pieces of tea leaves in each petri dish, and cultivate 10 petri dishes in total. The culture conditions are: 21°C, 10 hours of light, 14 hours of darkness, light Intensity 1200lux, relative humidity 85%, transfer to new callus culture medium with the same composition every 2 weeks for subculture; red callus begins to appear in the fourth week, wait until the red callus grows to a volum...

Embodiment 3

[0025] Select the vigorously growing "Jianbohuang" tea tree, take the third expanded leaf from each new shoot on the same tea tree, a total of 5 leaves, soak in 1% sodium hypochlorite aqueous solution for 19 minutes, take it out, and rinse with sterilized water Sodium hypochlorite solution, dried with sterile wind, under aseptic conditions, the leaves were cut into small pieces with an area of ​​0.8-1.2 square centimeters; the small pieces of leaves were transferred to callus culture medium (2.2g / L MS salt+0.15mg / L TDZ+0.08mg / L IBA+0.15mg / L GA 3 +20.0g / L sucrose+6g / L agar powder), put 10 small pieces of tea leaves in each petri dish, and cultivate 10 petri dishes in total. The culture conditions are: 21°C, 10 hours of light, 14 hours of darkness, light Intensity 1200lux, relative humidity 80%, transfer to new callus culture medium with the same composition every 2 weeks for subculture; red callus begins to appear in the fourth week, wait until the red callus grows to a volume...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting and preparing anthocyanin by using tea leaf callus suspension culture. The method comprises the steps as follows: third spread leaves on vigorous tea tree shoots are taken and cut into pieces after being sterilized, the pieces are cultured in a callus medium, the callus medium is replaced once every 2 weeks, the pieces are cut up when red callus appears and grows up after culture, the obtained substance is transferred to a suspension medium for vibration culture and then transferred to an induction medium for shake culture, suspension cultured cells are collected, dried and crushed, ultrasonic extraction is performed with a hydrochloric acid-ethanol solution, finally, vacuum concentration is performed until a solvent is completely volatized,and an anthocyanin product is obtained. An anthocyanin-containing tea leaf callus induction method, a suspension tissue culture method and a method for extracting anthocyanin from tea leaf suspensioncultured cells are established for the first time, and biological tissue with the anthocyanin content larger than 6% and far higher than that of any materials reported at present is obtained.

Description

technical field [0001] The invention relates to an extraction and preparation method of anthocyanin, in particular to a method for extracting and preparing anthocyanin by using tea callus suspension culture. Background technique [0002] Anthocyanin, also known as anthocyanin, is a kind of water-soluble natural pigment widely present in plants in nature, belonging to flavonoid compounds. Anthocyanins have good bioavailability in the human body, strong affinity to connective tissue, stable in acidic environment, long half-life, and long-lasting efficacy. Anthocyanins are powerful antioxidants that protect the body from harmful substances called free radicals, improve circulation and smooth the skin; help prevent many free radical-related diseases Diseases, including cancer, heart disease, enhances the elasticity of arteries, veins and capillaries; relieves hay fever and other allergies; acts as a barrier to protect brain cells from the formation of amyloid-beta protein, the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C07D311/62C09B61/00
CPCA01H4/001A01H4/008C07D311/62C09B61/00
Inventor 刘硕谦刘赛王若娴刘丽萍李志冰胡丹
Owner HUNAN AGRICULTURAL UNIV