Application of polyether type compound
A compound and polyether technology, applied in the field of medicinal chemistry and drug therapy, can solve the problems of drug resistance, toxic and side effects of tumor cells
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Embodiment 1
[0068] Biological method to obtain J1-001 sodium salt
[0069] Streptomyces nanchangensis subtype Streptomyces was inoculated on the SFM plate and cultured for about three to four days. When colonies are formed in the SFM medium, pick a single colony and culture it in the seed medium for about three to four days, and inoculate it into the fermentation medium according to 1% amount (the composition of the fermentation medium is 30g / L of soluble starch, yellow Bean cake powder 10g / L, yeast extract 2.5g / L, CaCO 3 3g / L, pH7.2). Culture conditions: 30 degrees, 220 rotation speed, culture for 7 to 8 days.
[0070] When the culture reaches the 7th or 8th day, collect the fermentation broth and centrifuge at a high speed to separate the supernatant from the mycelium, add an equal amount of acetone to the mycelium to destroy the bacteria, ultrasonicate for 20 minutes, spin evaporate acetone, use the supernatant and mycelium, etc. Amount of ethyl acetate extraction, repeated once. ...
Embodiment 2
[0073] To detect the inhibitory effect of J1-001 sodium salt on various tumor cells, the specific method is as follows:
[0074] (1) Cell recovery: Before the experiment, the ultra-clean workbench was irradiated with ultraviolet light for 30 minutes. Preheat the water bath to 37°C, and place the freshly prepared medium in the water bath to preheat. Take out the frozen cells, quickly put the frozen tube into the preheated water bath to thaw quickly, and shake it constantly to make the liquid in the tube melt quickly. After about 1 to 2 minutes, the liquid in the cryopreservation tube was completely dissolved, and the outer wall of the cryopreservation tube was wiped with a cotton ball containing 70% alcohol. Aspirate the cells in the cryopreservation tube, transfer to a 15ml centrifuge tube, and add 5ml of preheated complete medium at the same time. Centrifuge at a low speed of 500g for 3-5min, and discard the supernatant. Add 10ml of culture medium to the centrifuge tube, a...
Embodiment 3
[0087] J1-001 Sodium Salt Inhibits Breast Cancer Stem Cells
[0088] Taking Sanyin cell line MDA-MB-231 as the experimental object, breast cancer stem cells were enriched by suspension medium, and the inoculation density of breast cancer stem cells was 1000 cells / mL. Suspension culture medium (200ml) ingredients: B27 nutrient supplement factor 4mL; insulin 80μL; DMEM-F12 culture medium 194mL; human epidermal growth factor 40μL; The detection kit AldefluorTM kit was used for detection. The experimental results of adherent cells and suspension cells are as follows: figure 2 and 3 shown.
[0089] It can be seen that after about 12 days of suspension cell culture, the proportion of stem cells detected by flow cytometry in suspension culture increased to 25.12% (see Figure 4 ), compared to 3.97% of stem cells in adherent cells (see Figure 4 ), which can meet the requirements of subsequent experiments.
[0090] In order to increase the comparability between drugs, while mea...
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