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Magnetic bead time-resolved fluoroimmunoassy kit for quantitatively determining clenbuterol

A time-resolved fluorescence and immunoquantitative technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of inability to achieve rapid on-site detection, cumbersome detection process, and low accuracy, and achieve long fluorescence decay time, simple and fast operation. , the effect of a wide detection range

Inactive Publication Date: 2018-04-06
GUANGZHOU BIOKEY HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the above two detection methods are that the detection process is cumbersome, the detection time is long, expensive instruments are required, difficult to operate, and expensive
Enzyme-linked immunoassay has the advantages of accurate detection, high sensitivity, and simple equipment, but the detection time i

Method used

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  • Magnetic bead time-resolved fluoroimmunoassy kit for quantitatively determining clenbuterol
  • Magnetic bead time-resolved fluoroimmunoassy kit for quantitatively determining clenbuterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Magnetic bead time-resolved fluorescence immunoquantitative detection kit for clenbuterol, including BSA-CBL coated magnetic beads, fluorescent substance-labeled CBL antibody, washing solution, enhancing solution and clenbuterol standard solution.

[0030] The kit uses reagent strips for detection. The reagent strip consists of test well 1 (BSA-CBL coated carboxyl magnetic beads), marker well 3 (europium-labeled CBL antibody), washing solution wells 6 and 7, and enhancement solution well 12 ; The structure of the reagent strip is as figure 1 and figure 2 shown.

[0031] The preparation process of the magnetic bead time-resolved fluorescence immunoquantitative detection clenbuterol kit is as follows, wherein

[0032] (1) Preparation of BSA-CBL coated magnetic beads:

[0033] Wash beads

[0034] Draw 1mg of carboxyl magnetic beads with a diameter of 1μm into a 1.5ml centrifuge tube, place the centrifuge tube in a magnetic stand, use the magnetic stand to separate t...

Embodiment 2

[0062] Magnetic bead time-resolved fluorescence immunoquantitative detection kit for clenbuterol, including BSA-CBL coated magnetic beads, fluorescent substance-labeled CBL antibody, washing solution, enhancing solution and clenbuterol standard solution.

[0063] The kit uses reagent strips for detection. The reagent strip consists of the second well (test well 2) (BSA-CBL coated amino magnetic beads), the fourth well (marker well 4) (samarium-labeled CBL antibody), washing solution Holes 6 and 7, enhanced liquid hole composition, such as figure 1 and figure 2 shown.

[0064] The preparation process of the magnetic bead time-resolved fluorescence immunoquantitative detection clenbuterol kit is as follows, wherein

[0065] (1) Preparation of BSA-CBL coated magnetic beads:

[0066] ①Washing magnetic beads

[0067] Draw 1mg of 2μm amino magnetic beads into a 1.5ml centrifuge tube, place the centrifuge tube in the magnetic stand, use the magnetic stand to separate the magneti...

Embodiment 3

[0094] Example 3 Production and detection of reagent strips

[0095] The semi-finished reagent strips in this example are assembled through the following procedures: 50 μL of BSA-CBL-coated magnetic beads, 200 μL of samarium marker, and 900 μL of washing solution are separately packed in the first or second, third or fourth, sixth, seventh, and 12th , 900 μL of lotion, 300 μL of enhancement solution, and then seal it with a film sealing machine. The sealing film is coated with product information that can be scanned and identified by a fully automatic fluorescence detection analyzer, including enterprise standard curve, batch, production date, expiration date. The finished reagent strips, the packaged clenbuterol standard solution and other accessories are assembled into a kit.

[0096] Sample testing:

[0097] Loading

[0098] Put the sample to be tested or clenbuterol standard into the loading system of the automatic instrument, insert the reagent strip into the reagent...

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Abstract

The invention discloses a magnetic bead time-resolved fluoroimmunoassy kit for quantitatively determining clenbuterol. The kit is formed by a BSA-CBL coating magnetic bead, a fluorescent substance mark CBL antibody, a scrubbing solution, an enhancement solution and a clenbuterol standardized product solution. The sensitivity of the kit can reach 0.1ppb, the degree of precision is high, and the kithas no cross reaction with a clenbuterol structure and function analogue. Compared with a colloidal gold immunochromatograohic assay method, a fluorescence immunochromatography method and other methods, the magnetic bead time-resolved fluoroimmunoassy kit for quantitatively determining the clenbuterol has the advantages of high sensitivity and accuracy; compared with HPLC, GC/MS and ELISA, shortdetection time, low price, and capability of realizing field test of a large number of samples are achieved.

Description

technical field [0001] The invention relates to the field of food safety detection, in particular to a kit for quantitative detection of clenbuterol by magnetic beads time-resolved fluorescence immunoassay. Background technique [0002] Clenbuterol (Clenbuterol, CBL) is a β-adrenergic receptor agonist, mainly in the form of hydrochloride, adding Clenbuterol to feed can promote animal growth, inhibit fat deposition, and increase protein content , Increase ketone body lean meat rate, improve meat quality and so on. Therefore, in recent years, driven by economic interests, criminals have used a large amount of clenbuterol in animal husbandry and breeding. Excessive intake of CBL by the human body will lead to a series of adverse reactions, mainly muscle tremors, rapid heartbeat and breathing, etc., which can be life-threatening in severe cases. In view of this, many countries have banned the use of clenbuterol as feed and feed additives. In 2002, the Ministry of Agriculture...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/543G01N33/577
CPCG01N33/533G01N33/54326G01N33/577
Inventor 李根平
Owner GUANGZHOU BIOKEY HEALTH TECH CO LTD
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