Method used for discovering and identifying liver cancer serum differential expression proteins and verifying marker proteins

A technique for differentially expressed proteins and differentially expressed proteins, which is applied in the field of discovery and identification of differentially expressed proteins in liver cancer serum and verification marker proteins, which can solve the problems that the precision and accuracy cannot meet the analysis requirements and affect the quantitative detection of low-abundance proteins, etc.

Inactive Publication Date: 2018-04-10
南宁科城汇信息科技有限公司
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Problems solved by technology

The high sensitivity of mass spectrometry can help people discover low-abundance proteins and peptides in biological samples in the stage of marker discovery. The dynamic range greatly affects the quantitative detection of low-abundance proteins and peptides in biological fluids by mass spectrometry
Although researchers have tried to use a var

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  • Method used for discovering and identifying liver cancer serum differential expression proteins and verifying marker proteins
  • Method used for discovering and identifying liver cancer serum differential expression proteins and verifying marker proteins
  • Method used for discovering and identifying liver cancer serum differential expression proteins and verifying marker proteins

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[0021] Consists of the following steps:

[0022] Using iTRAQ labeling combined with MALDI-TOF MS / MS technology to discover and identify differentially expressed proteins in liver cancer serum, MRM verification marker proteins: using iTRAQ technology to analyze the serum of 30 cases of liver cancer and 30 cases of normal controls, a total of 304 proteins were detected (Confidence >95%), 38 differential proteins were found (Pfigure 1 It shows that one of the proteins Alpha-1-antitrypsin is highly expressed in liver cancer; from the 38 differential proteins found from the above iTRAQ markers, MIDAS (MRM Initiated Detection And Sequencing, MIDAS) technology was used to complete the MRM qualitative verification of 14 differential proteins; The standard method completed the MRM quantitative verification of three proteins, which not only fully proved the existence of these 14 differential proteins in the mixed sample, but also obtained the quantitative information of the three protein...

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Abstract

The invention discloses a method used for discovering and identifying liver cancer serum differential expression proteins and verifying marker proteins. According to the method, iTRAQ labeling MALDI-TOF MS/MS technology is adopted for discovering and identifying liver cancer serum differential expression proteins and MRM verifying of marker proteins. According to the method, searching on effectivetumor markers possesses important meaning on canceration mechanism study, disease early stage diagnosis, and prognosis. Selected novel liver cancer protein markers are subjected to in vivo and in vitro experiments such as gene knockin and knockout, and nude mice transplanted cancer, influences of blocking or up-regulating of expression of candidate genes on tumor biological behavior are observed,and key proteins capable of influencing human liver cancer occurrence and development are positioned, the effects of the key proteins in a system signal network is analyzed based on bioinformatics analysis, and the feasibility, the liver cancer diagnosis value, and the clinical significance of the key proteins in clinical applications including liver cancer prevention, early stage diagnosis, andprognosis is evaluated based on MRM absolute quantitative verification.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to a method for discovering and identifying differentially expressed proteins in liver cancer serum and verifying marker proteins. Background technique [0002] In the 20th century, primary liver cancer has risen to become the second cancer killer in my country, second only to lung cancer in urban areas and gastric cancer in rural areas [1] . At present, about 35,000 people in Guangxi die of malignant tumors every year, among which, about 12,000 people die of liver cancer. It can be seen that liver cancer is the first cancer killer in Guangxi. In Guangxi, the hazards of liver cancer are very uneven. The counties and cities with high mortality rate of liver cancer are mainly concentrated in the southwestern part of Guangxi. The three counties of Fusui, Long'an and Wuming around Nanning have the highest incidence. The incidence density of liver cancer in this loca...

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57438G01N33/57484G01N2800/60
Inventor 于思创王海云
Owner 南宁科城汇信息科技有限公司
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