A kind of creatine phosphate modified chitosan material and its preparation method and application
A technology of creatine phosphate and chitosan, which can be used in pharmaceutical formulations, prostheses, drug delivery, etc., can solve the problems of unsuitable phosphoric acid sources, easy to cause inflammation, etc., and achieve good bone conduction effect
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Embodiment 1
[0060] step one:
[0061] Dissolve 0.01 mol creatine phosphate in 100 ml MES buffer at pH 5, add activator 0.01 mol 1-ethyl-(3-dimethylaminopropylcarbodiimide) (EDC) and 0.01 mol N -Hydroxysuccinimide (NHS), react at room temperature for 20 min. The reacted solution was added dropwise to 100 ml chitosan-acetic acid solution with a volume concentration of 1v / v%, and reacted for 24 h at room temperature. The reaction solution was transferred to a dialysis bag with a molecular weight cut off of 7000, and the water was changed every 12 hours for 7 days of dialysis. The dialyzed solution was poured into a 18 cm glass petri dish and frozen at -80 °C for 12 h. The glass petri dish was placed in a lyophilizer with an ice condenser temperature of -50 °C and a vacuum degree of 0.4 for 2 days to obtain phosphocreatine-modified chitosan material (CS-PCr). figure 1 It is the NMR phosphophosphorus result of creatine phosphate modified chitosan (CS-PCr), which shows that creatine phosphat...
Embodiment 2
[0069] step one:
[0070] Dissolve 0.01 mol creatine phosphate in MES buffer at pH 5.5, add 0.05 mol 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC) and 0.05 mol N-hydroxybutanedi imide (NHS), react at room temperature for 20 min. The reacted solution was added dropwise to 100 ml chitosan-acetic acid solution with a volume fraction of 1v / v%, and reacted for 48 h at room temperature. The reaction solution was transferred to a dialysis bag with a molecular weight cut-off of 10,000, and the water was changed every 12 hours for 5 days of dialysis. The dialyzed solution was poured into a 18 cm glass petri dish and frozen at -80 °C for 12 h. The glass petri dish was placed in a lyophilizer with an ice condenser temperature of -50 °C and a vacuum degree of 0.7 mbar for 2 days to obtain phosphocreatine-modified chitosan (CS-PCr) material. The results of NMR-phosphorus spectrum of chitosan (CS-PCr) and figure 1 similar, indicating that phosphocreatine was successfully grafted ont...
Embodiment 3
[0078] step one:
[0079] Dissolve 0.01 mol creatine phosphate in 100 ml MES buffer at pH 6, add activator 0.1 mol 1-ethyl-(3-dimethylaminopropylcarbodiimide (EDC)) and 0.1 mol N -Hydroxysuccinimide (NHS), react at room temperature for 20 min. The reacted solution was added dropwise to 100 ml chitosan-acetic acid solution with a volume concentration of 1v / v%, and reacted at room temperature for 72 h. The reaction solution was transferred to a dialysis bag with a molecular weight cut-off of 13,000, and the water was changed every 12 h for 3 days of dialysis. The dialyzed solution was poured into a 18 cm glass petri dish and frozen at -80 °C for 12 h. The glass petri dish was placed in a lyophilizer with an ice condenser temperature of -50 °C and a vacuum of 1 mbar for 2 days to obtain phosphocreatine-modified chitosan (CS-PCr) material. Chitosan (CS-PCr) nuclear magnetic phosphorus spectrum results and figure 1 similar, indicating that phosphocreatine was successfully graft...
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