Method of producing a coffee extract
A coffee extraction and coffee technology, applied in the direction of coffee extraction, roasted coffee, coffee, etc., can solve the problems that have not been widely used
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[0024] A preferred embodiment of the method of the invention for the preparation of coffee extract comprises the following steps:
[0025] a) contacting roasted and ground coffee beans with an enzyme preparation comprising cellulase and xylanase; and
[0026] b) extracting said coffee beans with an aqueous liquid to prepare a coffee extract.
[0027] Another preferred embodiment of the method of the invention for the preparation of coffee extract comprises the following steps:
[0028] a) contacting roasted and ground coffee beans with an enzyme preparation comprising cellulase, xylanase, beta-mannanase and beta-glucosidase; and
[0029] b) extracting said coffee beans with an aqueous liquid to prepare a coffee extract.
Embodiment 1
[0031] Example 1: Hydrolysis of SGC with different enzymes
[0032] Coffee beans in the form of roasted and ground coffee bean granules that have undergone the conventional extraction process for the production of soluble coffee are subjected to enzymatic hydrolysis by different enzymes and combinations of enzymes.
[0033] Hydrolysis was performed at 30°C or 50°C for 15 minutes to 24 hours using 1% SGC in 50 mM maleate buffer (pH 5.0 or pH 7.0) and 50 μg / mL enzyme.
[0034] HPLC analysis of the sugars produced
[0035] To establish HPLC analysis, use Agilent Technology 1260 Infinity Quaternary LC system to detect different standard samples, the system is equipped with the following components: 1260ALS sampler, 1260 quaternary pump, 1260RID detector. Different columns (Hi-Plex Na and Hi-Plex H) were tested to determine the best separation. Water was used as the eluent at a flow rate of 0.5 mL for 1 minute.
[0036] The retention times (in minutes) of the following stand...
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