Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for using centrifugal tube plate

A technology of centrifuge tubes and tube sheets, which is applied in biochemical equipment and methods, microbiological measurement/inspection, DNA preparation, etc., which can solve the problems of time-consuming and labor-intensive consumption, large consumption of pipette tips, etc., and achieve shortened operation time and simplified operation steps , the effect of reducing the cost of testing

Inactive Publication Date: 2018-04-20
CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to ensure the full lysis of the pellet, it is necessary to fully mix the cell lysate and the pellet. However, because the sample is centrifuged at a high speed, the cell pellet is compacted. It is time-consuming and labor-intensive to mix by shaking. , and the consumption of gun head is relatively large

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments.

[0013] In this embodiment, the method of using the centrifuge tube plate provided by the present invention is described by using the extraction and sample loading process of the HPV DNA sample:

[0014] Take the samples to be tested and negative and positive quality control samples, fully equilibrate to room temperature, shake and mix.

[0015] After fully eluting the cervical brush, transfer 1mL of the eluate to a 1.5mL centrifuge tube.

[0016] If the sample preservation solution contains vegetation or wart tissue, a part should be taken into the 1.5mL centrifuge tube and recorded.

[0017] The 1.5 mL centrifuge tube was centrifuged at 13000 rpm for 10 minutes, the supernatant was discarded, and the cell pellet at the bo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for using a centrifugal tube plate. The method for using the centrifugal tube plate is about using the centrifugal tube plate for mixing materials in a centrifugal tube. The method for using the centrifugal tube plate solves the technical problem that a cell precipitation mixing method in the prior art consumes much time and effort and causes great tip loss.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for using a centrifugal tube plate. Background technique [0002] In the operation of DNA detection, there is a step of extracting nucleic acid samples. The commonly used method is to obtain a cell pellet after centrifugation, which is thoroughly mixed with the cell lysate and then boiled. The commonly used mixing method is to oscillate and mix on a vibrator, or to mix with a pipette tip. [0003] In order to ensure the full lysis of the pellet, it is necessary to fully mix the cell lysate and the pellet. However, because the sample is centrifuged at a high speed, the cell pellet is compacted. It is time-consuming and labor-intensive to mix by shaking. , and the consumption of gun head is relatively large. Contents of the invention [0004] In order to solve the technical problems of time-consuming and labor-intensive method of cell sedimentation and homogenizatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/10C12Q1/6806
CPCC12N15/1003C12Q1/6806
Inventor 唐笑田倩何媛李慧源周梅华彭兴旺刘岱璿
Owner CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST