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Bio-pharmaceutical application of three eupnane triterpenes to preparation of Gli gene inhibitor and medicine for preventing and curing liver cancer

A technology of biomedicine and inhibitor, applied in the field of biomedicine

Inactive Publication Date: 2018-05-01
陈世伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, there are no reports on the pharmacological activities and medicinal uses of Meliasenin B, C and D

Method used

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  • Bio-pharmaceutical application of three eupnane triterpenes to preparation of Gli gene inhibitor and medicine for preventing and curing liver cancer
  • Bio-pharmaceutical application of three eupnane triterpenes to preparation of Gli gene inhibitor and medicine for preventing and curing liver cancer
  • Bio-pharmaceutical application of three eupnane triterpenes to preparation of Gli gene inhibitor and medicine for preventing and curing liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Effects of Meliasenin B, C, and D on the proliferation of human liver cancer HepG2 cells

[0033] 1. Experimental materials

[0034] Human liver cancer HepG2 cell line was purchased from Shanghai Jikai Gene Technology Co., Ltd.;

[0035] DMEM medium, fetal bovine serum and PBS were purchased from Gibco Company; TRIzol was purchased from Invitrogen Company; the reverse transcription kit was purchased from Fermentas Company of the United States, and the fluorescent quantitative PCR kit was purchased from TaKaRabao Bioengineering (Dalian) Co., Ltd.; cell lysate , SDS-PAGE protein loading buffer (5×), BCA protein concentration test kit, 20×TPBS buffer, etc. were purchased from Jiangsu Biyuntian Biotechnology Research Institute, PVDF membrane was purchased from Millipore, USA; CCK-8 kit Purchased from Tongren Institute of Chemistry in Japan; Gli1, Gli2 and GAPDH primers were synthesized by Shanghai Sangon Bioengineering Company;

[0036] Rabbit anti-human Gli1 m...

Embodiment 2

[0071] Example 2: Effects of Meliasenin B, C, and D on the proliferation of human liver cancer SMMC-7721 line cells

[0072] 1. Experimental materials

[0073] Human liver cancer SMMC-7721 cell line was purchased from Shanghai Jikai Gene Technology Co., Ltd.;

[0074] DMEM medium, fetal bovine serum and PBS were purchased from Gibco Company; TRIzol was purchased from Invitrogen Company; the reverse transcription kit was purchased from Fermentas Company of the United States, and the fluorescent quantitative PCR kit was purchased from TaKaRabao Bioengineering (Dalian) Co., Ltd.; cell lysate , SDS-PAGE protein loading buffer (5×), BCA protein concentration test kit, 20×TPBS buffer, etc. were purchased from Jiangsu Biyuntian Biotechnology Research Institute, PVDF membrane was purchased from Millipore, USA; CCK-8 kit Purchased from Tongren Institute of Chemistry in Japan; Gli1, Gli2 and GAPDH primers were synthesized by Shanghai Sangon Bioengineering Company;

[0075] Rabbit anti...

Embodiment 3

[0109] Example 3: Effects of Meliasenin B, C, and D on growth of liver cancer xenografts in nude mice

[0110] HepG2 cells in a well-growth logarithmic growth phase were digested with trypsin, centrifuged and counted with trypan blue, and made 1×10 with normal saline 7 cells / mL of cell suspension were inoculated at 0.5 cm of the right axilla of nude mice, and each nude mouse was inoculated with 0.1 mL of cell suspension. Use a vernier caliper to measure the diameter of the transplanted tumor in nude mice, and wait until the tumor grows to about 100mm 3 At that time, the nude mice were randomly divided into Meliasenin B group, Meliasenin C group, Meliasenin D group and control group, with 10 mice in each group. At the same time, the nude mice in each group started to be administered. The administration plan of the Meliasenin B group, Meliasenin C group, and Meliasenin D group was: intragastric administration, 20 mg / kg / d, once a day, and the control group was given an equal vol...

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Abstract

The invention discloses bio-pharmaceutical application of three eupnane triterpenes to preparation of a Gli gene inhibitor and a medicine for preventing and curing the liver cancer. The bio-pharmaceutical application finds that Meliasenins B, C and D obviously inhibit the propagation of a HepG2 cell by inhibiting the expression of genes Gli1 and Gli2, the inhibition effect shows obvious dose dependence, and the Meliasenins B, C and D are effective inhibitors of the genes Gli1 and Gli2 and can be used to prepare the medicine for preventing and curing the liver cancer. The technician in the field knows that inhibiting the expression of the genes Gli1 and Gli2 is a relatively mature anti-cancer molecule passage, and the Meliasenins B, C and D play a role in resisting the liver cancer right through the passage. The bio-pharmaceutical application also finds that the naringenin reduces the toxicity of the Meliasenins B, C and D on the normal liver cell of people, but does not affect the toxicity of the Meliasenins B, C and D on the liver cancer cell.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to new applications of known compounds, in particular to the biomedical application of three euphorane-type triterpenes for the preparation of Gli gene inhibitors and drugs for preventing and treating liver cancer. Background technique [0002] Meliasenin B, C and D are three euphorane-type triterpenoids isolated from Toosendan (reference: Limonoids and Triterpenoids from the Stem Bark of Melia toosendan, J.Nat.Prod.2010,73,664-668) . [0003] [0004] Currently, there are no reports on the pharmacological activities and medical uses of Meliasenin B, C and D. However, some studies have shown that compounds in Toosendan, including but not limited to Meliasenin B, are related to the hepatotoxicity of Toosendan (Reference: Rapid Screening Research on Hepatotoxic Components in Toosendan, Chinese Journal of Traditional Chinese Medicine, Volume 38, Issue 11, June 2013). [0005] When screeni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/585A61K31/352A61P35/00
Inventor 陈世伟赖旭宇
Owner 陈世伟
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