Lactobacillus plantarum capable of adjusting ampicillin induced intestinal flora disturbance

A technology of Lactobacillus plantarum and Lactobacillus, applied in the direction of bacteria, microorganisms, milk substitutes, etc., can solve the problems of complex causes of intestinal diseases, no effective measures for long-term sub-health state, and insufficient symptoms, so as to improve the intestinal tract Function, simple preparation method, and high cell survival rate

Active Publication Date: 2018-05-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for easy production of bioactive substances like probiotics or other beneficial microorganisms that are effective at improving gut health by controlling their balance within specific areas called gastrointestinals (GI).

Problems solved by technology

This patent discusses how different substances like lutebile acids act differently when delivered into the colon due to their influence upon specific areas called niche where they could affect the entirety of the organism' s own ecology. These compounds help keep them alive within the gut without being absorbed from outside sources. Additionally, these agents targeted towards particular parts of the gut can enhance the natural defense against illnesses associated with indoor environments while minimizing damage to sensitive tissues during therapy procedures. Previous research suggests adding probioactive ingredients specifically designed to control intake levels and behavior of intraoral flora to provide benefits over time.

Method used

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  • Lactobacillus plantarum capable of adjusting ampicillin induced intestinal flora disturbance
  • Lactobacillus plantarum capable of adjusting ampicillin induced intestinal flora disturbance
  • Lactobacillus plantarum capable of adjusting ampicillin induced intestinal flora disturbance

Examples

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Embodiment 1

[0034] Embodiment 1: the determination of the growth generation time of different plant lactobacillus

[0035] The generation time refers to the time required for each cell division, and the specific growth rate is the largest in the logarithmic phase, so in the present invention, the bacterial generation time in the logarithmic growth phase of different Lactobacillus plantarum is measured under aerobic culture at 37°C.

[0036] The bacteria were inoculated in MRS medium with 2% inoculation amount, cultured at 37°C, and samples were taken every 0.5 h to measure the absorbance at 600 nm (OD600) of the bacteria solution. According to the law of bacterial growth, in the logarithmic growth period: A t =A 0 *2(t-0) / G, where A 0 Represents the initial number of bacteria when entering the logarithmic growth phase; A t Indicates the number of bacteria at time t; G represents the generation time of bacteria. Derivable formula: log 2 A t = log 2 A 0 +(1 / G)*t. It can be seen tha...

Embodiment 2

[0038] Embodiment 2: Determination of the survival rate of Lactobacillus plantarum in simulated gastrointestinal fluid in vitro

[0039] Prepare simulated gastrointestinal fluid, the simulated gastric juice is 6.2g / LNaCl, 2.2g / LKCl, 0.22g / L CaCl 2 , 1.2g / LNaHCO 3 , 0.3% pepsin, adjusted to pH 2.0 with 6M HCl; synthetic intestinal fluid 6.4g / LNaHCO 3 , 0.239 g / L KCl, 1.28 g / L NaCl and 0.1% pancreatin, adjusted to pH 7.4 with 6M HCl. After fully dissolving, filter and sterilize with a 0.22μm microporous membrane, dispense 5mL into each test tube, and refrigerate at 4°C for later use. Centrifuge the lactic acid bacteria cultured for 18 hours, resuspend and metabolize them with 0.9% normal saline for 1 hour, then insert them into the simulated gastric juice and mix them evenly, place them in an oscillating water bath at 37°C with a speed of 75r / min to simulate human gastrointestinal peristaltic digestion, and take them out after 2 hours . After centrifugation, simulated intest...

Embodiment 3

[0045] Example 3: Determination of Adhesion Performance of Different Lactobacillus plantarum to Human Colon Cancer Cell HT-29

[0046] HT-29 cells were cultured in RPMI 1640 (10% fetal bovine serum, 1% antibiotics) medium, and the medium was changed every two days until the cells reached 80-90%. Digest the cells with 0.25% trypsin solution and adjust the cell concentration to 1 x 10 5 cells / mL, and inoculated in a six-well culture plate, put a 18×18mm sterile coverslip in the culture plate in advance, and place it at 37°C, 95% air / 5% CO 2 Culture in an incubator until a dense monolayer of cells grows. After washing the cells twice with PBS buffer (pH 7.2), add 1 mL of antibiotic-free RPMI medium and 1 mL of cultured and resuspended bacteria suspension in PBS to each well (adjust the total number of bacteria to 10 8 ), mixed evenly and incubated in a 5% carbon dioxide incubator, each strain had three parallels. After culturing for 2 hours, the culture plate was taken out, an...

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Abstract

The invention discloses lactobacillus plantarum capable of adjusting ampicillin induced intestinal flora disturbance and belongs to the technical field of food. The lactobacillus plantarum CGMCC12436disclosed by the invention is amplification cultured under assistance of skimmed milk, trehalose and saccharose; after being frozen and dried, the lactobacillus plantarum is prepared into a biologicalpreparation; a viable bacterial content of the lactobacillus plantarum CGMCC12436 in the preparation is larger than 109CFU/g. The lactobacillus plantarum CGMCC12436 disclosed by the invention has theadvantages of lower growth generation time, high survival rate in in-vitro simulated gastric fluid and intestinal juice, good adhesion on human HT-29 cells and relatively lower drug resistance in drug allergy testing. The biological preparation prepared from the lactobacillus plantarum has the advantages that ampicillin induced intestinal flora disturbance in a mouse body can be obviously recovered, intestinal functions can be improved and has good market application prospect.

Description

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Claims

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Application Information

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Owner JIANGNAN UNIV
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