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SNP marker relevant with oil content of soybean seeds, zone, primer and application

A soybean seed and soybean oil technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of genetic improvement molecular marker-assisted selection breeding difficulties, undiscovered problems, etc., to reduce selection costs , improve efficiency, and accelerate the effect of the improvement process

Inactive Publication Date: 2018-05-04
JILIN ACAD OF AGRI SCI
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Problems solved by technology

However, no SNP (Single nucleotide polymorphism, single nucleotide polymorphism) molecular markers related to soybean oil content traits have been found, which brings difficulties to the genetic improvement of soybean seed oil content traits and molecular marker-assisted selection breeding

Method used

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  • SNP marker relevant with oil content of soybean seeds, zone, primer and application
  • SNP marker relevant with oil content of soybean seeds, zone, primer and application
  • SNP marker relevant with oil content of soybean seeds, zone, primer and application

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Embodiment Construction

[0022] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0023] Collected in the following examples and utilize the Soxhlet extraction method to measure the soybean germplasm resource information of oil content as follows: Including 280 soybean germplasm resources north of 40 ° N in my country, all provided by the Soybean Research Institute of Jilin Academy of Agricultural Sciences The cultivated soybean germplasm resources research team collected, evaluated, and stored in the germplasm resources bank of Jilin Academy of Agricultural Sciences. The sources of ...

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Abstract

The invention belongs to the technical field of soybean molecule biology, and particularly relates to an SNP marker relevant with soybean seed oil contents, a zone, a primer and application. The SNP marker is positioned at the 43173920th site of the No.18 chromosome of a soybean, and a nucleotide of the SNP marker is A or T; a nucleotide sequence of the marker sequence is shown in SEQ ID NO.1; a marker zone is ranged from the 43163920th site to the 43183920th site of the No.18 chromosome of the soybean. The SNP marker belongs to a codominant marker, is reliable and is convenient to use, so that a great convenience is provided for performing selective breeding on varieties of high oil contents of the soybeans, and the SNP marker can be applied to auxiliary selection of the molecular markerin improvement of soybean seed oil contents as well as fine location and map-based cloning of relevant genes of seed oil contents, so that an improved progress of the soybean seed oil content characters is accelerated.

Description

technical field [0001] The invention belongs to the technical field of soybean molecular biology, and specifically relates to a SNP marker, interval, primer and application related to soybean seed oil content. Background technique [0002] Soybean is an important oil crop, and its oil is an important source of food industry, industrial deep processing and medical health medicine. The content of unsaturated fatty acids in soybean oil is as high as 85%, among which linoleic acid and linolenic acid are essential fatty acids for the human body, which have important physiological functions such as lowering serum cholesterol and triglycerides and softening blood vessels, and are called "safe fatty acids" by nutritionists . Studies on the phenotypic identification of soybean oil have been reported for a long time. There are extensive genetic variations in oil content and fatty acid composition in soybean germplasm resources. Scientists have measured the oil content of 18,840 soy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王洋王跃强邱红梅侯云龙马晓萍于妍高淑芹陈健胡金海王丽
Owner JILIN ACAD OF AGRI SCI
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