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Method for simultaneously determining 12 sulfonamides in plasma by adopting ultra performance convergence chromatography

A high-efficiency convergence chromatography, sulfonamides technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of reducing the amount of use, high separation efficiency, and short separation time

Inactive Publication Date: 2018-05-04
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many analysis methods for SAs in biological samples at home and abroad, among which HPLC (UHPLC) detection technology is the most commonly used [GaoR, Zhang J, He X, et al.Anal Bioanal Chem.2010,398(1):451-461.Shaaban H ,GóreckiT.J ​​Sep Sci,2012,35(2):216-224.], SAs often have similar structures, no matter whether C18 column or amino column is adopted, the separation performance needs to be improved

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  • Method for simultaneously determining 12 sulfonamides in plasma by adopting ultra performance convergence chromatography
  • Method for simultaneously determining 12 sulfonamides in plasma by adopting ultra performance convergence chromatography
  • Method for simultaneously determining 12 sulfonamides in plasma by adopting ultra performance convergence chromatography

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Embodiment 1

[0041] 1 Materials and methods

[0042] 1.1 Instruments and reagents

[0043] Acquity UPC 2 (U.S. Waters Company), equipped with Waters PDA detector; Chromatographic column (both purchased from Waters, Milford, MA): (1) Waters ACQUITY UPC 2 BEH (1.7μm, 3×100mm I.D.), (2) Waters Acquity UPC 2 BEH 2-EP (1.7μm, 3×100mm I.D.), (3) Acquity UPC 2 CSHFluoro-Phenyl (1.7μm, 2.1×100mm I.D.) and (4) Acquity UPC 2HSS C18SB (1.8μm, 3×100mm I.D.). TOLEDOAL204 electronic balance (Switzerland METTLER company). The PURELAB Ultra MK2 ultrapure water meter was purchased from ELGA, UK.

[0044] SAs reference substances: sulfamethazine (SMZ, >99%, w / w), sulfadimethoxypyrimidine (SMM, >99%, w / w), sulfacetamide (Sulfacetamide, STD, >99%, w / w), sulfamerazine (Sulfamerazine, SMR, >99%, w / w), sulfadiazine (SDZ, >99%, w / w), sulfathiazole (Sulfathiazole, ST, >99%, w / w) and sulfapyridine (Sulfapyridine, SPD, >99%, w / w), were purchased from Dr. Ehrenstorfer, Germany. N 4 -Acesulfameoxazole (N 4 ...

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Abstract

The invention discloses a method for simultaneously separating and determining 12 sulfonamides in plasma by adopting ultra performance convergence chromatography (UPC2). The method comprises the following steps: adding acetonitrile into a plasma sample, performing vortex mixing, centrifuging, filtering the supernatant, determining by adopting the UPC2 method, adopting an Acquity UPC2TM BEH chromatographic column, taking CO2 and methanol as mobile phases, and performing gradient elution, wherein the temperature of a column oven is 40 DEG C, and the system back pressure is 2100psi. According tothe method disclosed by the invention, baseline separation of the 12 sulfonamides is realized within 6 minutes, the lowest limit of quantitation is 0.5 microg / mL, and the recovery rate is 80.53-103.33%. The method is simple and convenient to operate, accurate, has time-saving and environmental-friendly effects and is applicable to simultaneous detection of multiple sulfonamides in the plasma.

Description

technical field [0001] The invention relates to a method for simultaneously determining 12 kinds of sulfa drugs in blood plasma by using high-efficiency convergence chromatography. Background technique [0002] Sulfonamides (SAs) are a general term for a class of drugs containing sulfanilamide structure. Mainly by competing with p-aminobenzoic acid (PABA) to combine with dihydrofolate synthase, affecting the synthesis of bacterial nucleoproteins and exerting antibacterial effects. The drug has a wide antibacterial spectrum, low price and stable properties. advantage. So far, clinical application is still relatively extensive. [0003] Early studies have shown that taking the same dose of SAs drugs, there are large differences in blood drug concentrations in different patients. During conventional empirical treatment, the blood drug concentration is usually maintained at 50-100 μg / mL. In this range of blood drug concentration, the antibacterial effect of the drug is more ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/74G01N30/86
CPCG01N30/02G01N30/74G01N30/8634
Inventor 张元李国辉闫加庆刘敏沈鑫张远远刘佳周海燕
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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