Preparation method of electrophilic microbial preparation and application of electrophilic microbial preparation in treatment of uranium-containing wastewater

A microbial preparation and electrotaxis technology, which is applied in the field of screening and preparation of electrotaxis microorganisms, can solve the problems of less research on the electrotaxis of environmental microorganisms, and achieve the effects of easy industrial production, rapid removal, and environmental friendliness.

Inactive Publication Date: 2018-05-15
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many studies on electrotaxis of cells, but few studies on electrotaxis of environmental microorganisms

Method used

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  • Preparation method of electrophilic microbial preparation and application of electrophilic microbial preparation in treatment of uranium-containing wastewater
  • Preparation method of electrophilic microbial preparation and application of electrophilic microbial preparation in treatment of uranium-containing wastewater
  • Preparation method of electrophilic microbial preparation and application of electrophilic microbial preparation in treatment of uranium-containing wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method of the electrotactic microbial preparation of the present invention contains microbial strains and surfactants, and the microbial strains are obtained by screening and purifying the indigenous Laystia sp. species, inoculate the indigenous Layia bacterium cultivated to the logarithmic growth phase into the fermenter, add anionic surfactant, and adjust the content of the anionic surfactant to be 8%-10%, to obtain the electrotactic microbial preparation .

[0025] The number of live bacteria of indigenous Laysia in the microbial strains is 1×10 6 -10 8 individual / mL.

[0026] The anionic surfactant is selected from carboxylate, sulfonate, sulfate or phosphate surfactants, preferably alkyl phosphate.

Embodiment 2

[0028] The preparation method of the electrotactic microbial preparation of the present embodiment is different from that of Example 1 in that: the specific steps of screening, purifying and cultivating the indigenous Laysia species are as follows:

[0029] 1) Take 10 g of soil near a uranium mine in Hengyang City, add 90 ml of sterile water and mix well, let it stand at room temperature for 10 min, take the supernatant and make the concentration of 10 -1 to 10 -6 0.2 ml of different concentrations of bacterial suspensions were evenly spread on solid phosphorus-enriched plates, and cultured in a 30°C incubator for 48 h to obtain figure 1 the indicated strains;

[0030] Use an inoculation loop to pick a single colony and transfer it to a phosphorus-rich medium for repeated streaking, isolation and purification until the single colony on the plate has the same shape, and the obtained figure 2 The pure strain shown is white, moist and translucent in appearance.

[0031] The p...

Embodiment 3

[0034] This example is the application of the microbial preparation prepared by the method for preparing the electrotactic microbial preparation in the treatment of uranium-containing wastewater.

[0035] The biological agent of the present invention is used to treat metal UO in uranium-containing wastewater 2+ Ions, including the following steps: Take 100mL of low concentration UO with an initial concentration of 5 mg / L, 10 mg / L and 15 mg / L 2+ Solution, adjust the pH value of the solution to 6.0, add the electrotaxis microbial adsorbent prepared in Example 1, the consumption of this adsorbent is 0.25 g / L, carry out the adsorption reaction at 30 ℃ of constant temperature oscillator for 3 hours, then the Separation of sorbent from wastewater and determination of remaining UO in wastewater by split-tube photometry 2+ The content of the calculated adsorption capacity is shown in Table 1:

[0036] Table 1: Microbial preparations for different UO 2+ The adsorption capacity of wa...

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Abstract

The invention relates to a preparation method of an electrophilic microbial preparation and application of the electrophilic microbial preparation in treatment of uranium-containing wastewater. According to the preparation method of the electrophilic microbial preparation, a microbial strain and a surface active agent are used, and leifsonia polyphosphate is adopted as the microbial strain; the method comprises the steps of screening and purifying soil around an uranium tailings pond to obtain native leifsonia, culturing the native leifsonia for a logarithmic phase and then inoculating the cultured native leifsonia into a fermentation tank, adding an anionic surfactant, and adjusting the content of the anionic surfactant to 8-10% so as to obtain the electrophilic microbial preparation. Theinvention provides the microbial preparation which is green, cheap and practical and can be used for in-situ treatment and recovery of uranium in the uranium-containing wastewater. The electrophilicmicrobial preparation can effectively enhance the electrotaxis of microorganism and increase the uranium recovery efficiency.

Description

technical field [0001] The invention relates to a preparation method of a microbial preparation, in particular to a screening and preparation method of an electrotactic microorganism and a microorganism in-situ treatment and recovery technology for treatment and recovery of uranium-containing wastewater. Background technique [0002] A large amount of low-concentration uranium-containing wastewater is inevitably produced in the process of uranium mining and smelting, experiments, etc., of which mining and smelting wastewater accounts for the main body of the wastewater. The mass concentration of uranium in wastewater discharged from uranium mining, metallurgy and uranium processing is generally around 5 mg / L or below, which is about 125 times the allowable discharge concentration stipulated by the state and about 10,000 times the concentration in natural water bodies. In order to prevent the migration and diffusion of uranium, a cheap and effective treatment method is urgent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/01C02F101/20
CPCC02F3/34C02F2101/20C12N1/02C12N1/20
Inventor 谭文发丁蕾吕俊文王红强张晓文房琦
Owner NANHUA UNIV
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