A kind of preparation method of polypeptide-insulin nanoparticle
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A nanoparticle and insulin technology, applied in the field of biomedicine, can solve the problems of toxic immunogenicity and side effects, and achieve the effect of inhibiting insulin fibrosis and low cytotoxicity
Active Publication Date: 2019-12-10
SICHUAN UNIV
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However, these products need to overcome the problem of protein aggregation when they are applied clinically, because protein aggregation is easy to cause certain toxicity, immunogenicity and other side effects
Method used
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Embodiment 1
[0024] Prepare insulin monomer, polypeptide FVPRK, PBS buffer solution with a pH of 7.4, a 100ml round bottom flask, a magnetic stirring bar, and a constant temperature magnetic stirrer.
[0025] Weigh 12.5 mg of insulin monomer into a flask equipped with a stirrer, add 50 mL of PBS buffer solution with a pH of 7.4, and put it into a constant temperature magnetic stirrer with a well-adjusted temperature (37°C) at 200 r / min Stir, and take samples for testing at 0h, 1h, 2h, 3h, 4h, 5h, 6h to 25h.
Embodiment 2
[0027] Prepare commercial insulin, peptide FVPRK, PBS buffer solution with pH 7.4, 100ml round bottom flask, magnetic stir bar, constant temperature magnetic stirrer
[0028] Weigh 12.5 mg of insulin and put it into a flask equipped with a stirrer, add 50 mL of PBS buffer solution with a pH of 7.4, put it into a constant temperature magnetic stirrer with adjusted temperature (37°C), and stir at 200r / min , 0h, 1h, 2h, 3h, 4h, 5h, 6h to 25h sampling test.
Embodiment 3
[0030] Prepare insulin monomer, polypeptide FVPRK, PBS buffer solution with pH 7.4, 100ml round bottom flask, magnetic stirrer, constant temperature magnetic stirrer
[0031] Weigh 12.5 mg of insulin monomer and 6.9 mg of polypeptide FVPRK into a flask equipped with a stirring bar, add 50 mL of PBS buffer solution with a pH of 7.4, and put it into a constant temperature magnetic stirrer with adjusted temperature (37°C) Stir at 200r / min, and take samples for testing at 0h, 1h, 2h, 3h, 4h, 5h, 6h to 25h.
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Abstract
The invention discloses a preparation method of polypeptide-insulin nanoparticle. In the invention, insulin monomer is dissolved in a solution, and a certain proportion of polypeptide having the function of inhibiting insulin fibrosis is added at the same time; and then the above solution is stirred for a period of time under certain conditions. , so that the polypeptide and insulin are fully reacted to obtain pentapeptide-insulin supramolecular nanoparticles. The operation of the invention is simple, and the obtained supramolecular nanoparticles do not produce burst release, and have good sustained release effect, and the polypeptide can inhibit the fibrosis of insulin. This product has the potential to solve the dangers of hypoglycemia caused by the sudden release of insulin itself during clinical use and the problems of fibrosis during insulin use, and has broad application prospects in the treatment of type I diabetes and type II diabetes.
Description
technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a preparation method of polypeptide-insulin nanoparticles capable of inhibiting the fibrosis of insulin monomers, and has broad application prospects in the field of biomedicine for treating type I and type II diabetes. Background technique [0002] In the course of insulin treatment, due to the need to inject insulin every day, it has caused pain and inconvenience to patients. In order to overcome these problems in the course of treatment, various insulin administration routes have emerged: insulin analogs, insulin control release system, gel controlled release system and insulin pump. However, these products need to overcome the problem of protein aggregation when they are applied clinically, because protein aggregation is likely to cause certain toxicity, immunogenicity and other side effects. Therefore, a drug delivery system that can control the release and also has a ...
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