Enzyme and bacterium complex agent for harmless treatment of animal carcasses and preparation method of enzyme and bacterium complex agent

A technology for harmless treatment of animal carcasses, applied in biochemical equipment and methods, methods based on microorganisms, enzymes, etc., can solve problems such as inconvenience, low degradation efficiency, and complicated management

Active Publication Date: 2018-06-22
SHANDONG SUKAHAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are still problems such as time-consuming, complicated management, and low degradation efficiency in the method of using microorganisms to treat

Method used

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  • Enzyme and bacterium complex agent for harmless treatment of animal carcasses and preparation method of enzyme and bacterium complex agent
  • Enzyme and bacterium complex agent for harmless treatment of animal carcasses and preparation method of enzyme and bacterium complex agent
  • Enzyme and bacterium complex agent for harmless treatment of animal carcasses and preparation method of enzyme and bacterium complex agent

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preparation example Construction

[0043] The preparation method of the enzyme-bacteria compound agent for harmless treatment of animal corpses comprises the following steps:

[0044] Step 1, the fungus of Bacillus amyloliquefaciens, Bacillus cereus, Bacillus licheniformis, Candida lipolytica, Bacillus megaterium, Streptomyces subtropicale, Rhizopus oryzae, Sporosporium thermophila and Trichoderma viride were respectively Make dormant microorganism dry powder;

[0045] Step 2, mixing the dormant microorganism dry powder made by the various strains of the step 1 with protease and lipase in proportion to make the enzyme-bacteria compound agent;

[0046] Preferably, the preparation method of the dormant microorganism dry powder of the present invention comprises the following steps:

[0047] A. Activation culture

[0048] Respectively inoculate each of the strains into Erlenmeyer flasks filled with 15-30% activation medium by volume ratio for activation culture;

[0049] B. Inoculate the strains cultivated in the...

Embodiment 1

[0079] Embodiment 1: The weight ratio of each component is 30 parts of Bacillus amyloliquefaciens, 5 parts of Bacillus cereus, 8 parts of Bacillus licheniformis, 15 parts of subtropical Streptomyces, 10 parts of Candida lipolytica, 10 parts of Rhizopus oryzae 10 parts of Sporosporium thermophila, 3 parts of Bacillus megaterium, 4 parts of Trichoderma viridans, 2 parts of protease, 3 parts of lipase mixed with enzyme-bacteria compound agent for harmless treatment of dead animal corpses, fermented for 24 hours , the results obtained are: animal carcass weight reduction 92%, organic matter content of the material after fermentation is 53%, total nutrient 6%, water 28%, pH 6.9, roundworm egg mortality rate 100%, and no feces coliform bacteria.

Embodiment 2

[0080] Embodiment two: the weight ratio of each component is 15 parts of Bacillus amyloliquefaciens, 10 parts of Bacillus cereus, 4 parts of Bacillus licheniformis, 8 parts of subtropical Streptomyces, 20 parts of Candida lipolytica, 20 parts of Rhizopus oryzae 20 parts of Sporotrichum thermophila, 1 part of Bacillus megaterium, 2 parts of Trichoderma viridans, 0.5 parts of protease, 5 parts of lipase, the enzyme-bacteria compound agent made by mixing and harmless treatment of dead animal corpses, fermented for 36 hours , the results obtained are: animal carcasses reduced by 96%, the organic matter content of the material after fermentation is 55%, the total nutrient is 10%, the moisture is 20%, the pH is 7.2, the mortality rate of roundworm eggs is 100%, and no feces are detected coliform bacteria.

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Abstract

The invention relates to the technical field of harmless treatment of animal carcasses and provides an enzyme and bacterium complex agent for harmless treatment of animal carcasses. The enzyme and bacterium complex agent is prepared from bacillus amyloliquefaciens, bacillus cereus, bacillus licheniformis, streptomyces subtropicus, candida lipolytica, rhizopus oryzae, thermophilic sporotrichum, bacillus megaterium and trichoderma viride. Meanwhile, the invention also provides a preparation method of the enzyme and bacterium complex agent. The method comprises the steps as follows: firstly, allstrains are prepared into dry powder of hypopus microbes, the dry powder of each strain is mixed with protease and lipase in proportion, and the enzyme and bacterium complex agent is prepared. Thus, the bacillus amyloliquefaciens is screened from naturally fermented aerobic composted samples of animal carcasses from farms, the strain and strains of other components are prepared into the dry powderof hypopus microbes, the powder is mixed in a reasonable proportion according to applications, and the enzyme and bacterium complex agent for harmless treatment of died animal carcasses is obtained.

Description

technical field [0001] The invention relates to the technical field of harmless treatment of animal corpses, in particular to an enzyme-bacteria compound agent for harmless treatment of animal corpses and a preparation method thereof. Background technique [0002] With the rapid development of intensive and large-scale animal husbandry in my country, the amount of animal breeding has increased year by year, and the dead animal carcasses produced have caused huge pressure on environmental protection. Whether the harmless treatment of dead animal corpses is in place is not only related to the quality and safety of animal products and the health of the people, but also related to the sustainable development of animal husbandry and social public health safety. [0003] Taking Weifang City as an example, the city has large-scale farms with more than 100,000 households and more than 4,000 breeding communities. The city's annual slaughter of 10,000 pigs, 100,000 chickens, 100 dairy...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C12N9/50C12N9/20B09B3/00C12R1/07C12R1/085C12R1/10C12R1/465C12R1/73C12R1/645C12R1/11C12R1/885
CPCB09B3/00C12N1/14C12N1/16C12N1/20C12N9/20C12N9/50C12Y301/01003
Inventor 郭芳先肖发沂陈永科韩威华刘镇韩岳
Owner SHANDONG SUKAHAN BIO TECH
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