Molecular detection primers and molecular detection method of alternaria tenuissima pathogenic bacteria of leaf spot disease of potato plants

A technique for the detection of Alternaria spp. and molecules, which is applied in biochemical equipment and methods, measurement/inspection of microorganisms, DNA/RNA fragments, etc., can solve the problems of distinguishing various Alternaria genus, and achieve accurate identification Effect

Inactive Publication Date: 2018-06-22
黑龙江省农业科学院植物脱毒苗木研究所
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  • Abstract
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Problems solved by technology

[0004] The present invention aims at solving the problem that current traditional biological methods are aimed at potatoes, and it is difficult to distinguish various problems of Alternaria tenuissima among species, especially the problem of distinguishing Alternaria tenuissima (Alternaria tenuissima)

Method used

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  • Molecular detection primers and molecular detection method of alternaria tenuissima pathogenic bacteria of leaf spot disease of potato plants
  • Molecular detection primers and molecular detection method of alternaria tenuissima pathogenic bacteria of leaf spot disease of potato plants
  • Molecular detection primers and molecular detection method of alternaria tenuissima pathogenic bacteria of leaf spot disease of potato plants

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specific Embodiment approach 1

[0015] Embodiment 1: The primer sequences of this embodiment are shown in Seq ID No: 1 and Seq ID No: 2 in the sequence table.

specific Embodiment approach 2

[0016] Specific embodiment 2: The molecular detection method for the pathogenic bacteria of Alternaria thaliana for leaf spot disease of potato plants of the present embodiment is carried out according to the following steps:

[0017] 1. Take the potato genomic DNA to be detected as a template, and use the primers of claim 1 to carry out PCR amplification;

[0018] 2. Take the PCR product for gel electrophoresis detection, if a fragment of 240bp size can be detected, it can be judged that the detected potato has Alternaria parvum pathogenic bacteria.

specific Embodiment approach 3

[0019] Embodiment 3: This embodiment is different from Embodiment 2 in that the pathogenic bacteria of Alternaria tenuissima is Alternaria tenuissima. Others are the same as in the second embodiment.

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Abstract

The invention provides molecular detection primers and a molecular detection method of alternaria tenuissima pathogenic bacteria of leaf spot disease of potato plants, and relates to the field of potato detection. The invention aims at solving the problem that the current traditional biological method is difficult in distinguishing species of alternaria of potatoes; the primers provided by the invention are shown in Seq ID No. 1 and Seq ID No. 2; the primers can be used for carrying out polymerase chain reaction (PCR) detection of a sample to be detected. The method provided by the invention can rapidly and accurately identify the alternaria tenuissima, and has the detection sensitivity of 10pg / mu L.

Description

technical field [0001] The invention relates to the field of potato detection, in particular to molecular detection primers for pathogenic bacteria of the genus Alternaria tenuissima (Alternaria tenuissima), a leaf spot disease of potato plants, and a detection method thereof. Background technique [0002] At present, the total area and total output of potato in China ranks first in the world, but the yield per unit area is at a low level. The frequent occurrence of various diseases is one of the main reasons for the reduction of potato production. Among the potato diseases, fungal diseases have the most types, and are highly contagious and difficult to control. Some fungi can also mutate, posing a serious threat to potato production. Alternaria is an important fungal pathogen causing disease spots on potato plants and leaves. According to Zheng et al. (2015), there are three main types of Alternaria pathogens infecting Chinese potato plants: Alternaria solani, Alternaria t...

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/686C12Q1/04C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2531/113C12Q2565/125
Inventor 王晓丹吕典秋胡林双闵凡祥杨帅张静华高云飞魏琪董学志王文重马纪
Owner 黑龙江省农业科学院植物脱毒苗木研究所
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