Detecting method for residual amount of erythromycin in soil

A detection method and technology for erythromycin are applied in the field of detection of erythromycin residual titer in soil, which can solve the problems of inability to quickly and accurately detect erythromycin residue, low detection limit, low recovery rate, etc. High accuracy, reduced impact, and improved accuracy

Active Publication Date: 2018-06-22
NINGXIA HOPE FIELD BIOLOGICAL AGRI TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of low detection limit and low recovery rate in the prior art, which cannot quickly and accurately detect the residual amount of erythromycin in the soil, and there is no effective solution to the influence of the matrix effect, and a kind of soil is provided Detection method of erythromycin residue in

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  • Detecting method for residual amount of erythromycin in soil
  • Detecting method for residual amount of erythromycin in soil
  • Detecting method for residual amount of erythromycin in soil

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specific Embodiment approach 1

[0026] Specific embodiment one: the detection method of erythromycin residue in a kind of soil of the present embodiment, it is carried out according to the following steps:

[0027] 1. Sample preparation for the test product:

[0028] 1) Weigh the soil containing erythromycin, add extractant acetonitrile-Tris-CaCl 2 solution, vortexed for 45-60s, then assisted extraction in ultrasound for 20-30min, then centrifuged at 3500-4000rpm for 5-10min, took the supernatant, and collected the precipitate;

[0029] 2) Add extractant acetonitrile-Tris-CaCl to the precipitate collected in step 1) 2 solution, vortexed for 45-60s, then assisted extraction in ultrasound for 20-30min, then centrifuged at 3500-4000rpm for 5-10min, and took the supernatant;

[0030] 3) Combine the supernatants of step 1) and step 2), put them in a water bath at 35-40° C. and rotate to evaporate to one-fifth of the volume of the supernatant to obtain an inorganic phase;

[0031] 4) Add Tris-CaCl with a pH of ...

specific Embodiment approach 2

[0041] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the soil containing erythromycin and the extractant acetonitrile-Tris-CaCl 2 The mass volume ratio of the solution is 1g:15mL. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0042] Specific embodiment three: the difference between this embodiment and specific embodiment one is: precipitation and extraction agent acetonitrile-Tris-CaCl in step 2) 2 The mass volume ratio of the solution is 1g:15mL. Others are the same as in the first embodiment.

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Abstract

The invention discloses a detecting method for a residual amount of erythromycin in soil, and relates to a detecting method for residual titer of the erythromycin in the soil. The method comprises thefollowing steps: preparing a reference solution and preparing a test solution; then, injecting the reference solution and the test solution into a liquid chromatograph by adopting high performance liquid chromatography tandem mass spectrometry in a gradient eluting manner; calculating the titer of the erythromycin according to an internal standard method. According to the detecting method, the high performance liquid chromatography tandem mass spectrometry for the erythromycin in the soil is established by optimizing chromatographic conditions and testing test solution and reference solutionpreparation methods; the detecting method for the erythromycin in the soil is established by optimizing a chromatographic separating system; methodology validation test researches show that the established method is high in accuracy, strong in specificity and high in reproducibility, and can effectively detect the titer of the erythromycin in the soil.

Description

technical field [0001] The invention relates to the technical field of soil detection and testing, in particular to a detection method for erythromycin residue titer in soil. Background technique [0002] Trace amounts of antibiotics remain in the bacterial residue and degrade under the action of various factors, so the bacterial residue also contains its degradation products. If antibiotic residues enter the soil environment, the residual antibiotics and their degradation products will continue to accumulate, which will lead to the development of drug resistance genes in microorganisms, and the antibiotics will not be able to exert their antibacterial effect, seriously threatening human health. When antibiotic residues are used as fertilizers for vegetable cultivation, antibiotics may enter surface water and deeper soil with the erosion of rainwater, damaging the ecological environment. Antibiotics will also enter plants as they are absorbed by plants. Due to the action of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 王义张鹏金昌福李宁飞
Owner NINGXIA HOPE FIELD BIOLOGICAL AGRI TECH CO LTD
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