Method for building relevant characteristic fingerprint spectrum of dendrobium devonianum chemical micromolecular compositions

A fingerprint and Dendrobium toothpaste technology, applied in the field of fingerprint analysis and modeling, can solve problems such as high instrument requirements, inability to accurately detect macromolecular components, and inability to accurately evaluate the quality of Dendrobium Realize the effect of correlation analysis

Active Publication Date: 2018-06-26
北京蓝标一成科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In addition, in the existing Dendrobium detection standards, the polysaccharides and other macromolecular medicinal components are detected. However, the detection of polysaccharides and other macromolecular components require

Method used

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  • Method for building relevant characteristic fingerprint spectrum of dendrobium devonianum chemical micromolecular compositions
  • Method for building relevant characteristic fingerprint spectrum of dendrobium devonianum chemical micromolecular compositions
  • Method for building relevant characteristic fingerprint spectrum of dendrobium devonianum chemical micromolecular compositions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The extraction method of embodiment 1 dendrobium dendrobium

[0051] Take the dried sample of Dendrobium dendrobii, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder (weighing error cannot exceed 0.2%), place it in a 100ml Erlenmeyer flask, add 50mL 75 % Methanol (V 水 :V 甲醇 =25:75), took out after ultrasonic 30min at room temperature, filtered, and the filtrate was concentrated to dryness with 75% methanol solvent (V 水 :V 甲醇 =25:75) was dissolved, and finally transferred to a 10ml volumetric flask to constant volume, shaken up, and filtered with a 0.45 μm microporous membrane to obtain a Dendrobium dendrobium sample solution.

Embodiment 2

[0052] The chromatographic detection method of the dendrobium dendrobium extract of embodiment 2

[0053] 1. Sample extraction and processing methods for the determination of small molecule components in Dendrobium dendrobii:

[0054] Take 1.00g of the powder of this product (passed through the No. 3 sieve), accurately weigh it, put it in a 100ml volumetric flask, add 50ml of methanol-water (75:25) accurately, ultrasonicate (power 250W, frequency 40kHz) for 30 minutes, let cool, After filtration, the filtrate was concentrated to dryness by rotary evaporation, dissolved in 5ml of methanol-water (75:25), and the supernatant was passed through a 0.45μm microporous membrane, and the subsequent filtrate was obtained.

[0055] 2. Preparation of reference solution

[0056] Accurately weigh 4.10 mg of saffrontoside and 4.08 mg of naringenin respectively, place them in 10 ml volumetric flasks, add 75% methanol to dissolve and dilute, shake well, and use them as stock solutions. Refri...

Embodiment 3

[0067] The establishment of the correlation feature fingerprint spectrum with schaffertoside in Dendrobium dendrobium of embodiment 3

[0068] 1. Dendrobium sample solution preparation

[0069] Take the dry sample of Dendrobium, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder (the weighing error cannot exceed 0.2%), place it in a 100ml Erlenmeyer flask, add 50mL of 75% methanol ( V 水 :V 甲醇 =25:75), took out after ultrasonic 30min at room temperature, filtered, and the filtrate was concentrated to dryness with 75% methanol solvent (V 水 :V 甲醇 =25:75), and finally transferred to a 10ml volumetric flask to constant volume, shaken up, and filtered through a 0.45μm microporous membrane to obtain the product. Table 2 is the obtained linear relationship of the reference substance schaftoside.

[0070] Table 2 The linear relationship table of the reference substance Schaftoside

[0071]

[0072...

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Abstract

The invention discloses a method for building a relevant characteristic fingerprint spectrum of dendrobium devonianum chemical micromolecular compositions, the method comprises the following steps that (1) samples with the sample sizes of n are subjected to chromatographic detection respectively, and the chemical micromolecular composition schaftoside and/or naringenin are/is taken as detection data of reference ingredients; (2) the samples are subjected to fingerprint spectrum detection, and the fingerprint spectrum peak area value of the whole chemical composition of dendrobium devonianum isobtained; (3) the composition content of the schaftoside and/or naringenin in the spectrum data are/is taken as response variables, the peak area values of other compositions in the fingerprint spectrum are taken as independent variables to build an analysis model, and a micromolecular composition and fingerprint spectrum linear regression model is built; (4) the relevant characteristic fingerprint spectrum of the micromolecular compositions is built for the contribution degree of the regression model through the variable correlation coefficient index representation independent variables. According to the method for building the relevant characteristic fingerprint spectrum of the dendrobium devonianum chemical micromolecular compositions, the correlation analysis of the chemical micromolecular composition contents is realized, and the quality of the dendrobium devonianum medical material can be effectively identified, controlled and evaluated.

Description

technical field [0001] The invention belongs to the field of detection and analysis of traditional Chinese medicine components, and in particular relates to an analysis and modeling method for the fingerprint of dendrobium dendrobium. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium candidum, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which 76 are found in my country. Dendrobium has a long history of medicinal use. As early as in ancient times, Dendrobium was listed as a nourishing top grade in "Shen Nong's Materia Medica". For a long time, with the development of the times, Dendrobium has been regarded as a precious Chinese herbal medicine with very important nourishing effects. Dendrobium is clinically used to treat a variety of dis...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 邹婷婷邵自星
Owner 北京蓝标一成科技有限公司
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