Seed processing and seedling breeding method of artificial endosperm capsules of Chinese orchid

An artificial endosperm and seed technology, which is applied in the directions of seed coating/seed dressing, seed immunization, seed and rhizome treatment, etc., can solve the problems of no endosperm, high cost, incomplete embryo development, etc. Operation, low cost effect

Inactive Publication Date: 2018-06-29
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The seeds of Chinese orchid are very small, with a single seed weight of only 0.03-6.5 micrograms, and most of the embryos are not fully developed or have no endosperm, so the success rate of using seeds ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] S1. Seed collection: collect the fruit formed 150 days after artificial pollination when the orchid flowers are in full bloom, and take out the orchid seeds after the shell is damaged;

[0029] S2, seed coat damage: put the seeds collected in step S1 into a potassium hydroxide solution with a concentration of 0.05mol / L and soak for 3 minutes;

[0030] S3. Preparation of pretreatment solution: Dissolve glucose in 1% by weight in sterile purified water at 18°C, then add naphthaleneacetic acid dissolved in 5 times the weight of alcohol in advance to the solution, with a concentration of 100mg / L;

[0031] S4. Seed pretreatment: immerse the seeds treated in step S2 into the pretreatment solution prepared in step S3, and keep the temperature for 6 hours;

[0032] S5, seed disinfection: soak the seeds treated in step S4 in 0.1% potassium permanganate solution for 1 min and then take them out;

[0033] S6. Processing of artificial endosperm: After boiling tap water, add agar 5...

Embodiment 2

[0039] S1. Seed collection: collect the fruit formed 180 days after artificial pollination when the orchid flowers are in full bloom, and take out the orchid seeds after the shell is damaged;

[0040] S2, seed coat damage: put the seeds collected in step S1 into a potassium hydroxide solution with a concentration of 0.08mol / L and soak for 4 minutes;

[0041] S3. Preparation of pretreatment solution: dissolve glucose in 20°C sterile pure water according to the weight ratio of 2%, then add naphthaleneacetic acid dissolved in 7 times the weight of alcohol in advance to the solution, with a concentration of 300mg / L;

[0042] S4. Seed pretreatment: immerse the seeds treated in step S2 into the pretreatment solution prepared in step S3, and keep at a constant temperature for 8 hours;

[0043] S5, seed disinfection: soak the seeds treated in step S4 in 0.3% potassium permanganate solution for 2 minutes and then take them out;

[0044] S6. Processing of artificial endosperm: After bo...

Embodiment 3

[0050] S1. Seed collection: collect the fruit formed 210 days after artificial pollination when the orchid flowers are in full bloom, and take out the orchid seeds after the shell is damaged;

[0051] S2, seed coat damage: put the seeds collected in step S1 into a potassium hydroxide solution with a concentration of 0.1mol / L and soak for 5 minutes;

[0052] S3. Preparation of pretreatment solution: dissolve glucose in 3% by weight in sterile purified water at 25°C, then add naphthaleneacetic acid dissolved in 10 times the weight of alcohol in advance to the solution, with a concentration of 500 mg / L;

[0053] S4. Seed pretreatment: immerse the seeds treated in step S2 into the pretreatment solution prepared in step S3, and keep at a constant temperature for 12 hours;

[0054] S5, seed disinfection: soak the seeds treated in step S4 in 0.5% potassium permanganate solution for 3 minutes and then take them out;

[0055] S6. Processing of artificial endosperm: After boiling the t...

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PUM

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Abstract

The invention relates to a seed processing and seedling breeding method of artificial endosperm capsules of Chinese orchid. The method includes: S1, seed collection; S2, seed coat damaging; S3, pretreatment fluid configuration; S4, pretreatment of seeds; S5, seed disinfection; S6, artificial endosperm processing; S7, selection and processing of capsule shells; S8, capsule seed production; S9, seedling breeding; S10, maintenance. Endosperm composition of the Chinese orchid seeds is simulated, and nutrient substances needed for development of the seeds are artificially prepared and processed into the capsule seeds due to the reason that development of the Chinese orchid seeds is not perfect and endosperm is not fully formed resulting in that seedling breeding by the aid of the seeds is severely restricted, and the technical problem about low breeding success rate of the Chinese orchid due to the reason embryos are not well developed in the prior art is solved.

Description

technical field [0001] The invention relates to the processing and seedling raising method of orchid seeds, in particular to the processing and seedling raising method of orchid artificial endosperm capsule seeds. Background technique [0002] Guolan, also known as Chinese orchid, is the collective name of orchids originating in China, such as Chunlan, Jianlan, Molan, Hanlan, and Cymbidium. It is one of the most popular types of flowers and has high ornamental value and economic value. The seeds of Chinese orchid are very small, with a single seed weight of only 0.03-6.5 micrograms, and most of the embryos are not fully developed or have no endosperm, so the success rate of using seeds for reproduction is extremely low, while other reproduction methods are either costly or have high reproductive coefficients. low, resulting in serious restrictions on the production of orchid seedlings. Contents of the invention [0003] The purpose of the present invention is to provide ...

Claims

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Application Information

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IPC IPC(8): A01G22/63A01C1/08A01C1/06A01C1/00
CPCA01C1/00A01C1/06A01C1/08
Inventor 韩建秋周玉梅韩月
Owner SHANGHAI INST OF TECH
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